Human gut-derived commensal suppresses generation of T-cell response to gliadin in humanized mice by modulating gut microbiota

Rahul Bodkhe, Eric V. Marietta, Baskar Balakrishnan, David H. Luckey, Irina E. Horwath, Yogesh S. Shouche, Veena Taneja, Joseph A. Murray

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


The human intestinal tract is colonized by a large number of diverse microorganisms that play various important physiologic functions. In inflammatory gut diseases including celiac disease (CeD), a dysbiotic state of microbiome has been observed. Interestingly, this perturbed microbiome is normalized towards eubiosis in patients showing recovery after treatment. The treatment has been observed to increase the abundance of beneficial microbes in comparison to non-treated patients. In this study, we investigated the effect of Prevotella histicola or Prevotella melaninogenica, isolated from the duodenum of a treated CeD patient, on the induction and maintenance of oral tolerance to gliadin, a CeD associated subgroup of gluten proteins, in NOD.DQ8.ABo transgenic mice. Conventionally raised mice on a gluten free diet were orally gavaged with bacteria before and after injection with pepsin trypsin digested gliadin (PTD-gliadin). P. histicola suppressed the cellular response to gliadin, whereas P. melaninogenica failed to suppress an immune response against gliadin. Interestingly, tolerance to gliadin in NOD.DQ8.ABo mice may be associated with gut microbiota as mice gavaged with P melaninogenica harbored a different microbial diversity as compared to P. histicola treated mice. This study provides experimental evidence that gut microbes like P. histicola from treated patients can suppress the immune response against gliadin epitopes.

Original languageEnglish (US)
Article number102237
StatePublished - Apr 2021


  • Celiac disease
  • Gliadin
  • Gluten
  • Gut microbiota modulation
  • Oral tolerance
  • Prevotella histicola

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases


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