TY - JOUR
T1 - Human corneal endothelial cell transplantation in a human ex vivo model
AU - Patel, Sanjay V.
AU - Bachman, Lori A.
AU - Hann, Cheryl R.
AU - Bahler, Cindy K.
AU - Fautsch, Michael P.
PY - 2009
Y1 - 2009
N2 - PURPOSE. To determine the effects of incorporating superpara- magnetic microspheres (SPMs) into cultured human corneal endothelial cells (HCECs) and to describe preliminary experi- ments of HCEC transplantation, facilitated by SPMs and an external magnetic field, in a human anterior segment ex vivo model. METHODS. HCECs were cultured as monolayers and incorpo- rated with magnetite oxide SPMs (900, 300, and 100 nm) at different concentrations. Cell viability, migration toward a magnetic field, and light transmittance were measured after incorporation of the SPMs. HCEC transplantation into the eyes of human recipients was investigated by subjecting anterior segments in organ culture to an external magnetic field. Light and electron microscopy were used to assess HCEC attachment to corneal stroma. RESULTS. SPMs were incorporated into the cytoplasm of HCECs after overnight incubation. None of the SPMs affected the short-term viability of cultured HCECs (P 0.14, n 6) or their light transmittance (P 0.06, n 5), although there was a trend toward decreased transmittance with the higher concentration of 900-nm SPMs. Cell migration toward a magnetic field was higher for HCECs with incorporated SPMs than for HCECs without SPMs (P 0.01, n 6), with dose-response relationships evident for the 300- and 100-nm SPMs. SPMs facilitated the attachment of HCECs to the corneal stroma in the human anterior segment model with minimal change in intracameral (intraocular) pressure. CONCLUSIONS. SPMs facilitate migration of HCECs toward a magnetic source and attachment of cells to the corneal stroma without affecting cell viability or light transmittance. The human anterior segment model can be used to study HCEC transplantation.
AB - PURPOSE. To determine the effects of incorporating superpara- magnetic microspheres (SPMs) into cultured human corneal endothelial cells (HCECs) and to describe preliminary experi- ments of HCEC transplantation, facilitated by SPMs and an external magnetic field, in a human anterior segment ex vivo model. METHODS. HCECs were cultured as monolayers and incorpo- rated with magnetite oxide SPMs (900, 300, and 100 nm) at different concentrations. Cell viability, migration toward a magnetic field, and light transmittance were measured after incorporation of the SPMs. HCEC transplantation into the eyes of human recipients was investigated by subjecting anterior segments in organ culture to an external magnetic field. Light and electron microscopy were used to assess HCEC attachment to corneal stroma. RESULTS. SPMs were incorporated into the cytoplasm of HCECs after overnight incubation. None of the SPMs affected the short-term viability of cultured HCECs (P 0.14, n 6) or their light transmittance (P 0.06, n 5), although there was a trend toward decreased transmittance with the higher concentration of 900-nm SPMs. Cell migration toward a magnetic field was higher for HCECs with incorporated SPMs than for HCECs without SPMs (P 0.01, n 6), with dose-response relationships evident for the 300- and 100-nm SPMs. SPMs facilitated the attachment of HCECs to the corneal stroma in the human anterior segment model with minimal change in intracameral (intraocular) pressure. CONCLUSIONS. SPMs facilitate migration of HCECs toward a magnetic source and attachment of cells to the corneal stroma without affecting cell viability or light transmittance. The human anterior segment model can be used to study HCEC transplantation.
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U2 - 10.1167/iovs.08-2653
DO - 10.1167/iovs.08-2653
M3 - Article
C2 - 19136716
AN - SCOPUS:65549116506
SN - 0146-0404
VL - 50
SP - 2123
EP - 2131
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
IS - 5
ER -