@inbook{3ebace8e658f46e291eff7f1ff88770b,
title = "High-resolution characterization of DNA/protein complexes in living bacteria",
abstract = "The occurrence of DNA looping is ubiquitous. This process plays a well-documented role in the regulation of prokaryotic gene expression, such as the Escherichia coli lactose (lac) operon. Here, we present two complementary methods for high-resolution in vivo detection of DNA/protein binding within the bacterial nucleoid by using either chromatin immunoprecipitation combined with phage λ exonuclease digestion (ChIP-exo) or chromatin endogenous cleavage (ChEC), coupled with ligation-mediated polymerase chain reaction (LM-PCR) and Southern blot analysis. As an example we apply these in vivo protein-mapping methods to E. coli to show direct binding of architectural proteins in the Lac repressor-mediated DNA repression loop.",
keywords = "Architectural proteins, Chromatin endogenous cleavage (ChEC), Chromatin immunoprecipitation (ChIP), High-resolution mapping, Lac repression loop, Ligation-mediated PCR (LM-PCR), Phage lambda exonuclease, Polymerase chain reaction (PCR), Southern blot",
author = "Becker, {Nicole A.} and Peters, {Justin P.} and Maher, {L. James}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2018.",
year = "2018",
doi = "10.1007/978-1-4939-8675-0_6",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "95--115",
booktitle = "Methods in Molecular Biology",
}