TY - JOUR
T1 - High prevalence of tcdC deletion-carrying Clostridium difficile and lack of association with disease severity
AU - Verdoorn, Brandon P.
AU - Orenstein, Robert
AU - Rosenblatt, Jon E.
AU - Sloan, Lynne M.
AU - Schleck, Cathy D.
AU - Harmsen, William S.
AU - Nyre, Lisa M.
AU - Patel, Robin
N1 - Funding Information:
The authors gratefully acknowledge the Mayo Clinic Clinical Microbiology laboratory technologists, whose dedicated work made this study possible. This publication was supported by grant 1 UL1 RR024150-0 from the National Center for Research Resources , a component of the National Institutes of Health (NIH), and the NIH Roadmap for Medical Research, and by the Mayo Foundation. This study was presented, in part, at the 2008 American Society for Microbiology Annual Meeting.
PY - 2010/1
Y1 - 2010/1
N2 - We assessed the prevalence of tcdC deletion-carrying Clostridium difficile using a stool polymerase chain reaction (PCR) assay that detects previously described 18- and 39-bp deletions (J. Clin. Microbiol. 2008;46:1996). We divided inpatients into 2 groups, those for whom the assay detected a deletion in tcdC and those for whom no deletion was detected. We compared risk factors (antibiotic use, hospitalization, nursing home stay, immunocompromise, age >65 years), complications (pseudomembranous colitis, toxic megacolon, colonic perforation, colectomy, and intensive care unit admission), duration of antibiotic treatment, and 30-day mortality between the groups. Forty-two of 141 patients had deletion-positive C. difficile. Prior nursing home stay and age >65 years were significantly more common in the deletion-positive group. Other risk factors, complications, antibiotic duration, and mortality did not differ significantly. Deletion-carrying C. difficile was commonly present but not associated with more severe disease and not markedly different in terms of risk factor profile. Severity of disease was relatively low, regardless of the presence or absence of a deletion.
AB - We assessed the prevalence of tcdC deletion-carrying Clostridium difficile using a stool polymerase chain reaction (PCR) assay that detects previously described 18- and 39-bp deletions (J. Clin. Microbiol. 2008;46:1996). We divided inpatients into 2 groups, those for whom the assay detected a deletion in tcdC and those for whom no deletion was detected. We compared risk factors (antibiotic use, hospitalization, nursing home stay, immunocompromise, age >65 years), complications (pseudomembranous colitis, toxic megacolon, colonic perforation, colectomy, and intensive care unit admission), duration of antibiotic treatment, and 30-day mortality between the groups. Forty-two of 141 patients had deletion-positive C. difficile. Prior nursing home stay and age >65 years were significantly more common in the deletion-positive group. Other risk factors, complications, antibiotic duration, and mortality did not differ significantly. Deletion-carrying C. difficile was commonly present but not associated with more severe disease and not markedly different in terms of risk factor profile. Severity of disease was relatively low, regardless of the presence or absence of a deletion.
KW - Clostridium
KW - Difficile
KW - tcdC
UR - http://www.scopus.com/inward/record.url?scp=71049165929&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=71049165929&partnerID=8YFLogxK
U2 - 10.1016/j.diagmicrobio.2009.08.015
DO - 10.1016/j.diagmicrobio.2009.08.015
M3 - Article
C2 - 19775847
AN - SCOPUS:71049165929
SN - 0732-8893
VL - 66
SP - 24
EP - 28
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 1
ER -