Heteroduplex generator in analysis of Rh blood group alleles

Jay Stoerker; Carlyn Hurwitz; Nancy C. Rose; Leslie E. Silberstein; W. Edward Highsmith

doi:10.1093/clinchem/42.3.356

Heteroduplex generator in analysis of Rh blood group alleles

Jay Stoerker, Carlyn Hurwitz, Nancy C. Rose, Leslie E. Silberstein, W. Edward Highsmith

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

We describe the use of heteroduplex analysis to enhance the resolution of different rhesus-derived (Rh) isotypes. Heteroduplex analysis of different domains of the Rh D and Rh CE loci can be performed to diagnose a variety of blood group incompatibilities. One application of this technique is the ability to test for fetal-maternal blood group incompatibilities during pregnancy. Several new serotype-specific sequence variations were discovered in the mapping of the Rh locus, and used in the construction of artificial heteroduplex generators (HGs). HGs facilitate the resolution of the Rh isotypes by electrophoretic methods.

Original language	English (US)
Pages (from-to)	356-360
Number of pages	5
Journal	Clinical chemistry
Volume	42
Issue number	3
DOIs	https://doi.org/10.1093/clinchem/42.3.356
State	Published - 1996

Keywords

blood group incompatibilities
erythrocytes
fetal status
genetic variants
hemolytic disease of the newborn
immunology
newborns
pregnancy
serotype-specific sequence variations

ASJC Scopus subject areas

Clinical Biochemistry
Biochemistry, medical

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10.1093/clinchem/42.3.356

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title = "Heteroduplex generator in analysis of Rh blood group alleles",

abstract = "We describe the use of heteroduplex analysis to enhance the resolution of different rhesus-derived (Rh) isotypes. Heteroduplex analysis of different domains of the Rh D and Rh CE loci can be performed to diagnose a variety of blood group incompatibilities. One application of this technique is the ability to test for fetal-maternal blood group incompatibilities during pregnancy. Several new serotype-specific sequence variations were discovered in the mapping of the Rh locus, and used in the construction of artificial heteroduplex generators (HGs). HGs facilitate the resolution of the Rh isotypes by electrophoretic methods.",

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language = "English (US)",

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T1 - Heteroduplex generator in analysis of Rh blood group alleles

AU - Stoerker, Jay

AU - Hurwitz, Carlyn

AU - Rose, Nancy C.

AU - Silberstein, Leslie E.

AU - Highsmith, W. Edward

PY - 1996

Y1 - 1996

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AB - We describe the use of heteroduplex analysis to enhance the resolution of different rhesus-derived (Rh) isotypes. Heteroduplex analysis of different domains of the Rh D and Rh CE loci can be performed to diagnose a variety of blood group incompatibilities. One application of this technique is the ability to test for fetal-maternal blood group incompatibilities during pregnancy. Several new serotype-specific sequence variations were discovered in the mapping of the Rh locus, and used in the construction of artificial heteroduplex generators (HGs). HGs facilitate the resolution of the Rh isotypes by electrophoretic methods.

KW - blood group incompatibilities

KW - erythrocytes

KW - fetal status

KW - genetic variants

KW - hemolytic disease of the newborn

KW - immunology

KW - newborns

KW - pregnancy

KW - serotype-specific sequence variations

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Heteroduplex generator in analysis of Rh blood group alleles

Abstract

Keywords

ASJC Scopus subject areas

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