TY - JOUR
T1 - Hepatitis C virus NS5A protein protects against TNF-α mediated apoptotic cell death
AU - Ghosh, Asish K.
AU - Majumder, Mainak
AU - Steele, Robert
AU - Meyer, Keith
AU - Ray, Ranjit
AU - Ray, Ratna B.
N1 - Funding Information:
We thank C.M. Rice and K. Shimotohno for providing the NS5A plasmid DNA and monoclonal antibody to NS5A. This research was supported by PHS grant RO1AI45144 from the National Institutes of Health.
PY - 2000/4
Y1 - 2000/4
N2 - Hepatitis C virus (HCV) often causes a prolonged and persistent infection which may lead to hepatocellular carcinoma. We have previously reported that the nonstructural 5A (NS5A) protein of HCV promotes cell growth [Ghosh, A.K., Steele, R., Meyer, K., Ray, R., Ray, R.B., 1999. Hepatitis C virus NS5A protein modulates cell cycle regulatory genes and promotes cell growth. J. Gen. Virol. 80, 1179-1183]. In this study, we investigated the role of HCV NS5A (genotype 1a, strain H) in TNF-α induced apoptotic cell death. HepG2 cells expressing NS5A exhibited an inhibitory role in relation to TNF-α mediated apoptotic cell death. The NS5A protein blocked the activation of caspase-3 and inhibited proteolytic cleavage of the death substrate poly (ADP-ribose) polymerase in TNF-α induced cells. Together, these results suggest that HCV NS5A protein protects against TNF-α mediated apoptotic cell death. Copyright (C) 2000 Elsevier Science B.V.
AB - Hepatitis C virus (HCV) often causes a prolonged and persistent infection which may lead to hepatocellular carcinoma. We have previously reported that the nonstructural 5A (NS5A) protein of HCV promotes cell growth [Ghosh, A.K., Steele, R., Meyer, K., Ray, R., Ray, R.B., 1999. Hepatitis C virus NS5A protein modulates cell cycle regulatory genes and promotes cell growth. J. Gen. Virol. 80, 1179-1183]. In this study, we investigated the role of HCV NS5A (genotype 1a, strain H) in TNF-α induced apoptotic cell death. HepG2 cells expressing NS5A exhibited an inhibitory role in relation to TNF-α mediated apoptotic cell death. The NS5A protein blocked the activation of caspase-3 and inhibited proteolytic cleavage of the death substrate poly (ADP-ribose) polymerase in TNF-α induced cells. Together, these results suggest that HCV NS5A protein protects against TNF-α mediated apoptotic cell death. Copyright (C) 2000 Elsevier Science B.V.
KW - Apoptosis
KW - Hepatitis C virus
KW - TNF-α
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U2 - 10.1016/S0168-1702(00)00141-6
DO - 10.1016/S0168-1702(00)00141-6
M3 - Article
C2 - 10867196
AN - SCOPUS:0034084529
SN - 0168-1702
VL - 67
SP - 173
EP - 178
JO - Virus Research
JF - Virus Research
IS - 2
ER -