TY - JOUR
T1 - Helicobacter pylori infection affects Toll-like receptor 4 expression in human gastric mucosa
AU - Asahi, Kayoko
AU - Hai, Ying Fu
AU - Hayashi, Yujiro
AU - Eguchi, Hiroshi
AU - Murata, Hiroaki
AU - Tsujii, Masahiko
AU - Tsuji, Shingo
AU - Tanimura, Hirohisa
AU - Kawano, Sunao
PY - 2007/10
Y1 - 2007/10
N2 - Background/Aims: Toll-like receptor 4 (TLR4), which requires a helper molecule, MD-2, is a main receptor for lipopolysaccharide (LPS) from gram-negative bacteria. The expression of TLR4 in H. pylori infection in human gastric mucosa, however, is unclear. The aim of this study was to determine the effect of H. pylori infection on the TLR4 and MD-2 expression in human gastric mucosa. Methodology: Biopsy samples from the antrum and corpus were obtained from 45 patients (25 patients without H. pylori infection including 5 patients with successful eradication of H. pylori, and 20 patients with H. pylori infection). These samples were used for TLR4, MD-2 mRNA expression study and immunohistochemical study. Results: In patients without H. pylori infection, the expressions of TLR4 and MD-2 were bigger in the corpus mucosa than in the antral mucosa. In patients with H. pylori infection, the expressions of TLR4 and MD-2 significantly increased in the antral and corpus mucosa. Immunohistochemical study revealed similar results as the TLR4 mRNA expression. After the eradication of H. pylori, the expressions of TLR4 and MD-2 were the same levels in both sites as those in patients without H. pylori infection. Conclusions: The results indicated that H. pylori infection significantly increased TLE4 and MD-2 expressions in the antral and corpus mucosa.
AB - Background/Aims: Toll-like receptor 4 (TLR4), which requires a helper molecule, MD-2, is a main receptor for lipopolysaccharide (LPS) from gram-negative bacteria. The expression of TLR4 in H. pylori infection in human gastric mucosa, however, is unclear. The aim of this study was to determine the effect of H. pylori infection on the TLR4 and MD-2 expression in human gastric mucosa. Methodology: Biopsy samples from the antrum and corpus were obtained from 45 patients (25 patients without H. pylori infection including 5 patients with successful eradication of H. pylori, and 20 patients with H. pylori infection). These samples were used for TLR4, MD-2 mRNA expression study and immunohistochemical study. Results: In patients without H. pylori infection, the expressions of TLR4 and MD-2 were bigger in the corpus mucosa than in the antral mucosa. In patients with H. pylori infection, the expressions of TLR4 and MD-2 significantly increased in the antral and corpus mucosa. Immunohistochemical study revealed similar results as the TLR4 mRNA expression. After the eradication of H. pylori, the expressions of TLR4 and MD-2 were the same levels in both sites as those in patients without H. pylori infection. Conclusions: The results indicated that H. pylori infection significantly increased TLE4 and MD-2 expressions in the antral and corpus mucosa.
KW - Helicobacter pylori (H. pylori)
KW - MD-2
KW - Toll-like receptor 4 (TLR4)
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M3 - Article
C2 - 18251133
AN - SCOPUS:37749041203
SN - 0172-6390
VL - 54
SP - 1941
EP - 1944
JO - Hepato-Gastroenterology
JF - Hepato-Gastroenterology
IS - 79
ER -