Granulocyte mucopolysaccharide sulfation in patients with polycythemia vera

D. E. Babott, N. E. Kay, S. A. Landaw, A. J. Gottlieb

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Prior studies have revealed defective mucopolysaccharide sulfation (MPS) in leukemic granulocyte precursors. Polycythemia Vera (PV) is regarded as a chronic myeloproliferative disorder involving all cell lines of the bone marrow. Therefore, granulocyte MPS was studied in bone marrow samples obtained from 9 patients with PV, to determine whether a MPS defect exists in this disease. Thirteeen patients with unrelated diseases served as controls. Sulfation with 35SO4 was performed in short term bone marrow suspension culture employing a 3 hr incubation with isotope. Protein bound radioactivity was separated by gel filtration of the cell lysate and then counted. Results were expressed as cpm/107 cells incorporating isotope (myeloblasts, promyelocytes, as myelocytes). Values for MPS in controls ranged from 15,000 to 57,000, with a mean of 32,000 cpm/107 cells. Four patients with PV had normal MPS, while in 5 patients, MPS ranged from 2,000 to 14,000 cpm/107 cells. The defect in MPS in these 5 patients was significant when compared to controls (p<.001). MPS was correlated with clinical and chemical findings in the 9 PV patients. MPS did not correlate with Hgb, WBC, platelet count, red cell mass, serum B12, or B12 binding capacity. Four patients had normal leukocyte alkaline phosphatase (LAP) and low MPS; 4 had high LAP and normal MPS. One patient with low MPS and high LAP had been splenectomized prior to study. A defect in granulocyte mucopolysaccharide sulfation similar to that found in the granulocyte leukemias is therefore observed in polycythemia vera.

Original languageEnglish (US)
Pages (from-to)268A
JournalClinical Research
Issue number3
StatePublished - 1975

ASJC Scopus subject areas

  • General Medicine


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