TY - JOUR
T1 - Genetic analysis of SIGMAR1 as a cause of familial ALS with dementia
AU - Belzil, Véronique V.
AU - Daoud, Hussein
AU - Camu, William
AU - Strong, Michael J.
AU - Dion, Patrick A.
AU - Rouleau, Guy A.
N1 - Funding Information:
VVB and HD are supported by the Canadian Institutes of Health Research. We would like to thank the patients and the families involved in this study, and to acknowledge support from the Association pour la Recherche sur la Sclérose Latérale Amyotrophique (ARS), the Association Franc¸aise contre les Myopathies (AFM), and the French Group on MND. GAR holds the Canada’s Research Chair in Neurogenetics and a Jeanne-et-J.-Louis-Levesque Chair for the Genetics of Brain Diseases.
PY - 2013/2
Y1 - 2013/2
N2 - Amyotrophic lateral sclerosis (ALS) is the most common motor neuron diseases (MND), while frontotemporal lobar degeneration (FTLD) is the second most common cause of early-onset dementia. Many ALS families segregating FTLD have been reported, particularly over the last decade. Recently, mutations in TARDBP, FUS/TLS, and C9ORF72 have been identified in both ALS and FTLD patients, while mutations in VCP, a FTLD associated gene, have been found in ALS families. Distinct variants located in the 3′-untranslated region (UTR) of the SIGMAR1 gene were previously reported in three unrelated FTLD or FTLD-MND families. We directly sequenced the coding and UTR regions of the SIGMAR1 gene in a targeted cohort of 25 individual familial ALS cases of Caucasian origin with a history of cognitive impairments. This screening identified one variant in the 3′-UTR of the SIGMAR1 gene in one ALS patient, but the same variant was also observed in 1 out of 380 control chromosomes. Subsequently, we screened the same samples for a C9ORF72 repeat expansion: 52% of this cohort was found expanded, including the sample with the SIGMAR1 3′-UTR variant. Consequently, coding and noncoding variants located in the 3′-UTR region of the SIGMAR1 gene are not the cause of FTLD-MND in our cohort, and more than half of this targeted cohort is genetically explained by C9ORF72 repeat expansions.
AB - Amyotrophic lateral sclerosis (ALS) is the most common motor neuron diseases (MND), while frontotemporal lobar degeneration (FTLD) is the second most common cause of early-onset dementia. Many ALS families segregating FTLD have been reported, particularly over the last decade. Recently, mutations in TARDBP, FUS/TLS, and C9ORF72 have been identified in both ALS and FTLD patients, while mutations in VCP, a FTLD associated gene, have been found in ALS families. Distinct variants located in the 3′-untranslated region (UTR) of the SIGMAR1 gene were previously reported in three unrelated FTLD or FTLD-MND families. We directly sequenced the coding and UTR regions of the SIGMAR1 gene in a targeted cohort of 25 individual familial ALS cases of Caucasian origin with a history of cognitive impairments. This screening identified one variant in the 3′-UTR of the SIGMAR1 gene in one ALS patient, but the same variant was also observed in 1 out of 380 control chromosomes. Subsequently, we screened the same samples for a C9ORF72 repeat expansion: 52% of this cohort was found expanded, including the sample with the SIGMAR1 3′-UTR variant. Consequently, coding and noncoding variants located in the 3′-UTR region of the SIGMAR1 gene are not the cause of FTLD-MND in our cohort, and more than half of this targeted cohort is genetically explained by C9ORF72 repeat expansions.
KW - Amyotrophic lateral sclerosis
KW - dementia
KW - frontotemporal lobar degeneration
KW - motor neuron disease
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U2 - 10.1038/ejhg.2012.135
DO - 10.1038/ejhg.2012.135
M3 - Article
C2 - 22739338
AN - SCOPUS:84872493515
SN - 1018-4813
VL - 21
SP - 237
EP - 239
JO - European Journal of Human Genetics
JF - European Journal of Human Genetics
IS - 2
ER -