Genetic analysis of CD28 signaling

Tiffani A. Greene, Virginia Smith Shapiro

Research output: Contribution to journalReview articlepeer-review

5 Scopus citations


T cell activation is central to initiating an immune response. Two signals are required: an antigen-specific signal through the T cell receptor (TCR) and an antigen-independent costimulatory signal, primarily through CD28 in naïve T cells. Although many of the molecules involved in TCR signal transduction have been identified, the signaling pathways downstream of CD28 involved in costimulation are not well-defined. Through mutagenesis, we have generated a panel of Jurkat T cell lines in which CD28 costimulation fails to upregulate the RE/AP composite element of the IL-2 promoter. Biochemical analysis and genetic rescue of the defects in these cell lines will lead to a better understanding of CD28 signal transduction.

Original languageEnglish (US)
Pages (from-to)513-520
Number of pages8
JournalImmunologic Research
Issue number2-3
StatePublished - 2003


  • CD28
  • RE/AP
  • Signal transduction

ASJC Scopus subject areas

  • Immunology


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