Generation and validation of APOE knockout human iPSC-derived cerebral organoids

Yuka A. Martens; Siming Xu; Richard Tait; Gary Li; Xinping C. Zhao; Wenyan Lu; Chia Chen Liu; Takahisa Kanekiyo; Guojun Bu; Jing Zhao

doi:10.1016/j.xpro.2021.100571

Generation and validation of APOE knockout human iPSC-derived cerebral organoids

Yuka A. Martens, Siming Xu, Richard Tait, Gary Li, Xinping C. Zhao, Wenyan Lu, Chia Chen Liu, Takahisa Kanekiyo, Guojun Bu, Jing Zhao

Neuroscience

Research output: Contribution to journal › Article › peer-review

Abstract

Apolipoprotein E (apoE) is a major lipid carrier in the brain and closely associated with the pathogenesis of Alzheimer's disease (AD). Here, we describe a protocol for efficient knockout of APOE in human induced pluripotent stem cells (iPSCs) using the CRISPR-Cas9 system. We obtain homozygous APOE knockout (APOE^-/-) iPSCs and further validate the deficiency of apoE in iPSC-derived cerebral organoids. APOE^-/- cerebral organoids can serve as a useful tool to study apoE functions and apoE-related pathogenic mechanisms in AD. For complete details on the use and execution of this protocol, please refer to Zhao et al. (2020).

Original language	English (US)
Article number	100571
Journal	STAR Protocols
Volume	2
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2021.100571
State	Published - Jun 18 2021

Keywords

CRISPR
Neuroscience
Organoids
Stem Cells

ASJC Scopus subject areas

General Immunology and Microbiology
General Biochemistry, Genetics and Molecular Biology
General Neuroscience

Access to Document

10.1016/j.xpro.2021.100571

Cite this

@article{c57c2d70b61047838bb60c30ab11ab33,

title = "Generation and validation of APOE knockout human iPSC-derived cerebral organoids",

abstract = "Apolipoprotein E (apoE) is a major lipid carrier in the brain and closely associated with the pathogenesis of Alzheimer's disease (AD). Here, we describe a protocol for efficient knockout of APOE in human induced pluripotent stem cells (iPSCs) using the CRISPR-Cas9 system. We obtain homozygous APOE knockout (APOE-/-) iPSCs and further validate the deficiency of apoE in iPSC-derived cerebral organoids. APOE-/- cerebral organoids can serve as a useful tool to study apoE functions and apoE-related pathogenic mechanisms in AD. For complete details on the use and execution of this protocol, please refer to Zhao et al. (2020).",

keywords = "CRISPR, Neuroscience, Organoids, Stem Cells",

author = "Martens, {Yuka A.} and Siming Xu and Richard Tait and Gary Li and Zhao, {Xinping C.} and Wenyan Lu and Liu, {Chia Chen} and Takahisa Kanekiyo and Guojun Bu and Jing Zhao",

note = "Publisher Copyright: {\textcopyright} 2021 The Author(s)",

year = "2021",

month = jun,

day = "18",

doi = "10.1016/j.xpro.2021.100571",

language = "English (US)",

volume = "2",

journal = "STAR Protocols",

issn = "2666-1667",

publisher = "Cell Press",

number = "2",

}

TY - JOUR

T1 - Generation and validation of APOE knockout human iPSC-derived cerebral organoids

AU - Martens, Yuka A.

AU - Xu, Siming

AU - Tait, Richard

AU - Li, Gary

AU - Zhao, Xinping C.

AU - Lu, Wenyan

AU - Liu, Chia Chen

AU - Kanekiyo, Takahisa

AU - Bu, Guojun

AU - Zhao, Jing

PY - 2021/6/18

Y1 - 2021/6/18

N2 - Apolipoprotein E (apoE) is a major lipid carrier in the brain and closely associated with the pathogenesis of Alzheimer's disease (AD). Here, we describe a protocol for efficient knockout of APOE in human induced pluripotent stem cells (iPSCs) using the CRISPR-Cas9 system. We obtain homozygous APOE knockout (APOE-/-) iPSCs and further validate the deficiency of apoE in iPSC-derived cerebral organoids. APOE-/- cerebral organoids can serve as a useful tool to study apoE functions and apoE-related pathogenic mechanisms in AD. For complete details on the use and execution of this protocol, please refer to Zhao et al. (2020).

AB - Apolipoprotein E (apoE) is a major lipid carrier in the brain and closely associated with the pathogenesis of Alzheimer's disease (AD). Here, we describe a protocol for efficient knockout of APOE in human induced pluripotent stem cells (iPSCs) using the CRISPR-Cas9 system. We obtain homozygous APOE knockout (APOE-/-) iPSCs and further validate the deficiency of apoE in iPSC-derived cerebral organoids. APOE-/- cerebral organoids can serve as a useful tool to study apoE functions and apoE-related pathogenic mechanisms in AD. For complete details on the use and execution of this protocol, please refer to Zhao et al. (2020).

KW - CRISPR

KW - Neuroscience

KW - Organoids

KW - Stem Cells

UR - http://www.scopus.com/inward/record.url?scp=85107299124&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85107299124&partnerID=8YFLogxK

U2 - 10.1016/j.xpro.2021.100571

DO - 10.1016/j.xpro.2021.100571

M3 - Article

C2 - 34151296

AN - SCOPUS:85107299124

SN - 2666-1667

VL - 2

JO - STAR Protocols

JF - STAR Protocols

IS - 2

M1 - 100571

ER -

Generation and validation of APOE knockout human iPSC-derived cerebral organoids

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this