TY - JOUR
T1 - Gene transfer to arteries.
AU - Akyürek, Levent M.
AU - San, Hong
AU - Nabel, Elizabeth G.
AU - Singh, Ripudamanjit
AU - Simari, Robert D.
PY - 2002/2
Y1 - 2002/2
N2 - This unit provides a set of protocols for introducing recombinant genes into normal, injured, and atherosclerotic arteries. The protocols include animal preparation, surgical techniques, and delivery systems. Protocols describe gene delivery to normal, injured, and stented porcine iliofemoral arteries, employing a double balloon infusion catheter to deliver the vector. Another basic protocol describes gene delivery to atherosclerotic arteries using a hyperlipidemic double-injury rabbit model, and requires surgical exposure of the artery and instillation of the gene vector via a catheter. Additional protocols describe gene delivery to normal and injured murine carotid and femoral arteries. An Alternate Protocol describes a percutaneous method for arterial gene delivery. These protocols may be adapted to deliver genes to either injured or noninjured atherosclerotic arteries.
AB - This unit provides a set of protocols for introducing recombinant genes into normal, injured, and atherosclerotic arteries. The protocols include animal preparation, surgical techniques, and delivery systems. Protocols describe gene delivery to normal, injured, and stented porcine iliofemoral arteries, employing a double balloon infusion catheter to deliver the vector. Another basic protocol describes gene delivery to atherosclerotic arteries using a hyperlipidemic double-injury rabbit model, and requires surgical exposure of the artery and instillation of the gene vector via a catheter. Additional protocols describe gene delivery to normal and injured murine carotid and femoral arteries. An Alternate Protocol describes a percutaneous method for arterial gene delivery. These protocols may be adapted to deliver genes to either injured or noninjured atherosclerotic arteries.
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M3 - Article
C2 - 18428325
AN - SCOPUS:44049096168
SN - 1934-8266
VL - Chapter 13
SP - Unit13.1
JO - Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]
JF - Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]
ER -