Gallotannin suppresses calcium oxalate crystal binding and oxalate- induced oxidative stress in renal epithelial cells

Hyo Jung Lee, Soo Jin Jeong, Moon Nyeo Park, Michael Linnes, Hee Jeoung Han, Jin Hyoung Kim, John Charles Lieske, Sung Hoon Kim

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


Calcium oxalate monohydrate (COM) crystals bind avidly to the surface of proliferating and migrating renal endothelial cells, perhaps a key event in kidney stone formation. Oxalate-induced pre-oxidative stress can further promote crystal attachment cells. Natural products including gallotannins found in green teas have been studied as potentially novel treatments to prevent crystal retention and kidney stone formation. Gallotannin significantly inhibited COM crystal growth and binding to Madin-Darby Canine Kidney Cells type I (MDCK I) renal epithelial cells at non-toxic concentrations. Reverse transcription polymerase chain reaction (RT-PCR) analysis revealed that gallotannin significantly attenuated oxalate-induced mRNA and protein expressions of monocyte chemoattractant protein 1 (MCP-1), osteopontin (OPN), nicotinamide adenine dinucleotide phosphate (NADPH) oxidase subunit p22 phox and p47 phox in human primary renal epithelial cells (HRCs). Gallotannin also reduced the levels of reactive oxygen species (ROS) and malondialdehyde (MDA) as well as enhanced antioxidant enzyme superoxide dismutase (SOD) activity in oxalate treated HRCs. Taken together, our findings suggest that gallotannin can contribute to nephrolithiasis prevention via direct effects on renal epithelial cells including suppression of COM binding and MCP-1 and OPN expression, along with augmenting antioxidant activity.

Original languageEnglish (US)
Pages (from-to)539-544
Number of pages6
JournalBiological and Pharmaceutical Bulletin
Issue number4
StatePublished - Apr 2012


  • Calcium oxalate monohydrate
  • Gallotannin
  • Monocyte chemoattractant protein 1
  • Osteopontin
  • Reactive oxygen species
  • Renal epithelial cell

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science


Dive into the research topics of 'Gallotannin suppresses calcium oxalate crystal binding and oxalate- induced oxidative stress in renal epithelial cells'. Together they form a unique fingerprint.

Cite this