Abstract
Dendritic cells (DCs) are major antigen-presenting cells that can efficiently prime and cross-prime antigen-specific T cells. Delivering antigen to DCs via surface receptors is thus an appealing strategy to evoke cellular immunity. Nonetheless, which DC surface receptor to target to yield the optimal CD8 + and CD4 + T cell responses remains elusive. Herein, we report the superiority of CD40 over 9 different lectins and scavenger receptors at evoking antigen-specific CD8 + T cell responses. However, lectins (e.g., LOX-1 and Dectin-1) were more efficient than CD40 at eliciting CD4 + T cell responses. Common and distinct patterns of subcellular and intracellular localization of receptor-bound αCD40, αLOX-1 and αDectin-1 further support their functional specialization at enhancing antigen presentation to either CD8 + or CD4 + T cells. Lastly, we demonstrate that antigen targeting to CD40 can evoke potent antigen-specific CD8 + T cell responses in human CD40 transgenic mice. This study provides fundamental information for the rational design of vaccines against cancers and viral infections.
Original language | English (US) |
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Pages (from-to) | 46-58 |
Number of pages | 13 |
Journal | EBioMedicine |
Volume | 5 |
DOIs | |
State | Published - Mar 1 2016 |
Keywords
- CD40
- Cross-presentation
- Dendritic cell
- Lectins
- Vaccine
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology