Fkbp1a controls ventricular myocardium trabeculation and compaction by regulating endocardial Notch1 activity

Hanying Chen, Wenjun Zhang, Xiaoxin Sun, Momoko Yoshimoto, Zhuang Chen, Wuqiang Zhu, Jijia Liu, Yadan Shen, Weidong Yong, Deqiang Li, Jin Zhang, Yang Lin, Baiyan Li, Nathan J. VanDusen, Paige Snider, Robert J. Schwartz, Simon J. Conway, Loren J. Field, Mervin C. Yoder, Anthony B. FirulliNadia Carlesso, Jeffrey A. Towbin, Weinian Shou

Research output: Contribution to journalArticlepeer-review

50 Scopus citations


Trabeculation and compaction of the embryonic myocardium are morphogenetic events crucial for the formation and function of the ventricular walls. Fkbp1a (FKBP12) is a ubiquitously expressed cis-trans peptidyl-prolyl isomerase. Fkbp1a-deficient mice develop ventricular hypertrabeculation and noncompaction. To determine the physiological function of Fkbp1a in regulating the intercellular and intracellular signaling pathways involved in ventricular trabeculation and compaction, we generated a series of Fkbp1a conditional knockouts. Surprisingly, cardiomyocyte-restricted ablation of Fkbp1a did not give rise to the ventricular developmental defect, whereas endothelial cell-restricted ablation of Fkbp1a recapitulated the ventricular hypertrabeculation and noncompaction observed in Fkbp1a systemically deficient mice, suggesting an important contribution of Fkbp1a within the developing endocardia in regulating the morphogenesis of ventricular trabeculation and compaction. Further analysis demonstrated that Fkbp1a is a novel negative modulator of activated Notch1. Activated Notch1 (N1ICD) was significantly upregulated in Fkbp1a-ablated endothelial cells in vivo and in vitro. Overexpression of Fkbp1a significantly reduced the stability of N1ICD and direct inhibition of Notch signaling significantly reduced hypertrabeculation in Fkbp1a-deficient mice. Our findings suggest that Fkbp1a-mediated regulation of Notch1 plays an important role in intercellular communication between endocardium and myocardium, which is crucial in controlling the formation of the ventricular walls.

Original languageEnglish (US)
Pages (from-to)1946-1957
Number of pages12
JournalDevelopment (Cambridge)
Issue number9
StatePublished - May 1 2013


  • Endocardial-myocardial signaling
  • FK506 binding protein 12
  • Mouse
  • Notch1
  • Ventricular hypertrabeculation/noncompaction

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology


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