Fitness of cell-mediated immunity independent of repertoire diversity

Mouhammed AbuAttieh, Michelle Rebrovich, Peter J. Wettstein, Zvezdana Vuk-Pavlovic, Andrew H. Limper, Jeffrey L. Platt, Marilia Cascalho

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Fitness of cell-mediated immunity is thought to depend on TCR diversity; however, this concept has not been tested formally. We tested the concept using JH-/- mice that lack B cells and have TCR Vβ diversity <1% that of wild-type mice and quasi-monoclonal (QM) mice with oligoclonal B cells and TCR Vβ diversity 7% that of wild-type mice. Despite having a TCR repertoire contracted >99% and defective lymphoid organogenesis, JH -/- mice rejected H-Y-incompatible skin grafts as rapidly as wild-type mice. JH-/- mice exhibited T cell priming by peptide and delayed-type hypersensitivity, although these responses were less than normal owing either to TCR repertoire contraction or defective lymphoid organogenesis. QM mice with TCR diversity contracted >90%, and normal lymphoid organs rejected H-Y incompatible skin grafts as rapidly as wild type mice and exhibited normal T cell priming and normal delayed-type hypersensitivity reactions. QM mice also resisted Pneumocystis murina like wild-type mice. Thus, cell-mediated immunity can function normally despite contractions of TCR diversity >90% and possibly >99%.

Original languageEnglish (US)
Pages (from-to)2950-2960
Number of pages11
JournalJournal of Immunology
Issue number5
StatePublished - Mar 1 2007

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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