@article{0c47bdfa7e8e4bb88923b7c09879dfa1,
title = "Fibrin glue mediated delivery of bone anabolic reagents to enhance healing of tendon to bone",
abstract = "Tendon graft healing in bone tunnels for the fixation of intra-articular ligament reconstructions may limit clinical outcome by delaying healing. This study assesses the effects of hydrogel-mediated delivery of bone anabolic growth factors in a validated model of tendon-to-bone tunnel healing. Forty-five Wistar rats were randomly allocated into three groups (BMP2-treated, GSK126-treated, and placebo). All animals underwent a tendon-to-bone tunnel reconstruction. Healing was evaluated at 4 weeks by biomechanical assessment, micro-computed tomography (bone mineral density, bone volume, cross sectional area of bone tunnels), and traditional histology. Adverse events associated with the hydrogel-mediated delivery of drugs were not observed. Results of our biomechanical assessment demonstrated favorable trends in animals treated with bone anabolic factors for energy absorption (P = 0.116) and elongation (P = 0.054), while results for force to failure (P = 0.691) and stiffness (P = 0.404) did not show discernible differences. Cross sectional areas for BMP2-treated animals were reduced, but neither BMP2 nor GSK126 administration altered bone mineral density (P = 0.492) or bone volume in the bone tunnel. These results suggest a novel and positive effect of bone anabolic factors on tendon-to-bone tunnel healing. Histological evaluation confirmed absence of collagen fibers crossing the soft tissue-bone interface indicating immature graft integration as expected at this time point. Our study indicates that hydrogel-mediated delivery of BMP2 and GSK126 appears to be safe and has the potential to enhance tendon-to-bone tunnel healing in ligament reconstructions.",
keywords = "BMP2, GSK126, connective tissue, ligament reconstruction, orthopedic, tendon-to-bone healing",
author = "Endre Soreide and Denbeigh, {Janet M.} and Lewallen, {Eric A.} and Samsonraj, {Rebekah M.} and Berglund, {Lawrence J.} and Amel Dudakovic and Cool, {Simon M.} and Lars Nordsletten and Sanjeev Kakar and {van Wijnen}, {Andre J.}",
note = "Funding Information: National Institutes of Health, Grant numbers: F32 AR068154, R01 AR049069, F32 AR066508; Research Council of Norway, Grant number: 239871/F20; Robert and Arlene Kogod Center on Aging; Mayo Clinic Materials and Structural Testing Research Core Funding Information: We thank Phillip Edwards for assistance with μCT image analysis and interpretation. Jenny Pattengill and Ronald Marler facilitated histological assessment. In addition, members of the van Wijnen laboratory contributed valuable expertise to our animal experiments. This work was supported in part by The Research Council of Norway 239871/F20 (ES) and National Institutes of Health, R01 AR049069 (AVW), F32 AR068154 (EAL), F32 AR066508 (AD), as well as a Career Development Grant from the Robert and Arlene Kogod Center on Aging, Mayo Clinic (RMS). This work was also supported by the Mayo Clinic Materials and Structural Testing Research Core. Funding Information: We thank Phillip Edwards for assistance with ?CT image analysis and interpretation. Jenny Pattengill and Ronald Marler facilitated histological assessment. In addition, members of the van Wijnen laboratory contributed valuable expertise to our animal experiments. This work was supported in part by The Research Council of Norway 239871/F20 (ES) and National Institutes of Health, R01 AR049069 (AVW), F32 AR068154 (EAL), F32 AR066508 (AD), as well as a Career Development Grant from the Robert and Arlene Kogod Center on Aging, Mayo Clinic (RMS). This work was also supported by the Mayo Clinic Materials and Structural Testing Research Core. Publisher Copyright: {\textcopyright} 2018 Wiley Periodicals, Inc.",
year = "2018",
month = jul,
doi = "10.1002/jcb.26755",
language = "English (US)",
volume = "119",
pages = "5715--5724",
journal = "Journal of cellular biochemistry",
issn = "0730-2312",
publisher = "Wiley-Liss Inc.",
number = "7",
}