Factors in standardizing automated cholinesterase assays

Barry W. Wilson, Stephanie Padilla, John D. Henderson, Stephan Brimijoin, Proveen D. Dass, Glenn Elliot, Bruce Jaeger, David Lanz, Renee Pearson, Rachel Spies

Research output: Contribution to journalArticlepeer-review

40 Scopus citations


A scientific panel assembled by the U.S. Environmental Protection Agency (EPA) determined that variability in cholinesterase (ChE) activities in the agency's pesticide/animal study database likely was due to a lack of accepted guidelines for ChE methodology. A series of trials was held in which participating laboratories measured ChE activity in blood and brain samples from untreated and pesticide-treated rats using a colorimetric assay method. The degree of inhibition of ChE activity in plasma and brain samples compared to controls was consistent among most of the laboratories. The ChE activity in erythrocyte samples differed more between laboratories due to a high blank, low erythrocyte AChE activity and hemoglobin absorption at the wavelength of the assay. Strategies are suggested for minimizing the variability of ChE activity in hemoglobin-rich samples.

Original languageEnglish (US)
Pages (from-to)187-196
Number of pages10
JournalJournal of Toxicology and Environmental Health
Issue number2
StatePublished - Apr 1996

ASJC Scopus subject areas

  • Toxicology
  • Pollution


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