TY - CHAP
T1 - Extracellular Flux Analysis of Mitochondrial Function in Pluripotent Stem Cells
AU - Tsogtbaatar, Enkhtuul
AU - Minter-Dykhouse, Katherine
AU - Saarinen, Alicia
AU - Folmes, Clifford D.L.
N1 - Publisher Copyright:
© 2022, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022
Y1 - 2022
N2 - Mitochondrial function and energy metabolism are increasingly recognized not only as regulators of pluripotent stem cell function and fate, but also as critical targets in disease pathogenesis and aging. Therefore across the downstream applications of pluripotent stem cells, including development and disease modeling, drug screening, and cell-based therapies, it is crucial to be able to measure mitochondrial function and metabolism in a high-throughput, real-time and label-free manner. Here we describe the application of Seahorse extracellular flux analysis to measure mitochondrial function in pluripotent stem cells and their derivatives. Specifically, we highlight two assays, the Mitochondrial Stress Test, which quantifies overall mitochondrial function including basal, maximal and ATP-couple oxygen consumption rates, and the Electron Transport Chain Complex Specific assay, that quantifies function of individual complexes within the electron transport chain.
AB - Mitochondrial function and energy metabolism are increasingly recognized not only as regulators of pluripotent stem cell function and fate, but also as critical targets in disease pathogenesis and aging. Therefore across the downstream applications of pluripotent stem cells, including development and disease modeling, drug screening, and cell-based therapies, it is crucial to be able to measure mitochondrial function and metabolism in a high-throughput, real-time and label-free manner. Here we describe the application of Seahorse extracellular flux analysis to measure mitochondrial function in pluripotent stem cells and their derivatives. Specifically, we highlight two assays, the Mitochondrial Stress Test, which quantifies overall mitochondrial function including basal, maximal and ATP-couple oxygen consumption rates, and the Electron Transport Chain Complex Specific assay, that quantifies function of individual complexes within the electron transport chain.
KW - Differentiation
KW - Embryonic stem cells
KW - Induced pluripotent stem cells
KW - Mitochondrial respiration
KW - Oxidative metabolism
KW - Oxidative phosphorylation
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U2 - 10.1007/978-1-0716-1979-7_7
DO - 10.1007/978-1-0716-1979-7_7
M3 - Chapter
C2 - 35507157
AN - SCOPUS:85129432840
T3 - Methods in Molecular Biology
SP - 85
EP - 102
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -