Abstract
As an alternative to manual assays that track insulin secretion, we tested a silicon-based biosensor that allows automated monitoring of extracellular acidification. Glucose stimulation of INS-1 and HIT-T15 cells resulted in a rapid increase in extracellular acidification in a biphasic and concentration-dependent fashion much like insulin secretion (EC50 INS-1 = 5 mM and HIT-T15 = 1 mM). This response was attenuated by verapamil (10 μM) and stimulated by administration of glybenclamide (100 nM) or KCl-induced (40 mM) depolarization. These experiments suggest that automated monitoring of extracellular pH may be a useful assay and support the relevance of linking metabolic activity to insulin secretion.
Original language | English (US) |
---|---|
Pages (from-to) | 1168-1173 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 293 |
Issue number | 4 |
DOIs | |
State | Published - 2002 |
Keywords
- Biphasic
- Cytosensor
- Diabetes
- Microphysiometry
- pH
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology