Expression of the AML-1 oncogene shortens the G1 phase of the cell cycle

David K. Strom, Nip John, Jennifer J. Westendorf, Bryan Linggi, Bart Lutterbach, James R. Downing, Noel Lenny, Scott W. Hiebert

Research output: Contribution to journalArticlepeer-review

87 Scopus citations


The AML-1-encoded transcription factor, AML-1B, regulates numerous hematopoietic-specific genes. Inappropriate expression of AML-1-family proteins is oncogenic in cell culture systems and in mice. To understand the oncogenic functions of AML-1, we established cell lines expressing AML-1B to examine the role of AML-1 in the cell cycle. DNA content analysis and bromodeoxyuridine pulse-chase studies indicated that entry into the S phase of the cell cycle was accelerated by up to 4 h in AML-1B-expressing 32D.3 myeloid progenitor cells as compared with control cells or cells expressing E2F-1. However, AML-1B was not able to induce continued cell cycle progression in the absence of growth factors. The DNA binding and transactivation domains of AML-1B were required for altering the cell cycle. Thus, AML-1B is the first transcription factor that affects the timing of the mammalian cell cycle.

Original languageEnglish (US)
Pages (from-to)3438-3445
Number of pages8
JournalJournal of Biological Chemistry
Issue number5
StatePublished - Feb 4 2000

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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