Expression of SH2D2A in T-cells is regulated both at the transcriptional and translational level

Kristin Melkevik Kolltveit; Stine Granum; Hans Christian Aasheim; Monika Forsbring; Vibeke Sundvold-Gjerstad; Ke Zheng Dai; Øyvind Molberg; Karoline W. Schjetne; Bjarne Bogen; Virginia S. Shapiro; Finn Eirik Johansen; Karl Schenck; Anne Spurkland

doi:10.1016/j.molimm.2007.11.005

Expression of SH2D2A in T-cells is regulated both at the transcriptional and translational level

Kristin Melkevik Kolltveit, Stine Granum, Hans Christian Aasheim, Monika Forsbring, Vibeke Sundvold-Gjerstad, Ke Zheng Dai, Øyvind Molberg, Karoline W. Schjetne, Bjarne Bogen, Virginia S. Shapiro, Finn Eirik Johansen, Karl Schenck, Anne Spurkland

Immunology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The T-cell specific adapter protein (TSAd) encoded by the SH2D2A gene is up-regulated in activated human CD4+ T-cells in a cAMP-dependent manner. Expression of SH2D2A is important for proper activation of T-cells. Here, we show that SH2D2A expression is regulated both at the transcriptional and translational level. cAMP signaling alone induces TSAd-mRNA expression but fails to induce increased TSAd protein levels. By contrast, TCR engagement provides signals for both TSAd transcription and translation. We further show that cAMP signaling can prime T-cells for a more prompt expression of TSAd protein upon TCR stimulation. Our study thus points to a novel mechanism for how cAMP signaling may modulate T-cell activation through transcriptional priming of resting cells.

Original language	English (US)
Pages (from-to)	2380-2390
Number of pages	11
Journal	Molecular Immunology
Volume	45
Issue number	8
DOIs	https://doi.org/10.1016/j.molimm.2007.11.005
State	Published - Apr 2008

Keywords

Adapter protein
Gene expression regulation
T-cell activation
cAMP

ASJC Scopus subject areas

Immunology
Molecular Biology

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10.1016/j.molimm.2007.11.005

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Kolltveit, K. M., Granum, S., Aasheim, H. C., Forsbring, M., Sundvold-Gjerstad, V., Dai, K. Z., Molberg, Ø., Schjetne, K. W., Bogen, B., Shapiro, V. S., Johansen, F. E., Schenck, K., & Spurkland, A. (2008). Expression of SH2D2A in T-cells is regulated both at the transcriptional and translational level. Molecular Immunology, 45(8), 2380-2390. https://doi.org/10.1016/j.molimm.2007.11.005

Kolltveit, KM, Granum, S, Aasheim, HC, Forsbring, M, Sundvold-Gjerstad, V, Dai, KZ, Molberg, Ø, Schjetne, KW, Bogen, B, Shapiro, VS, Johansen, FE, Schenck, K & Spurkland, A 2008, 'Expression of SH2D2A in T-cells is regulated both at the transcriptional and translational level', Molecular Immunology, vol. 45, no. 8, pp. 2380-2390. https://doi.org/10.1016/j.molimm.2007.11.005

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title = "Expression of SH2D2A in T-cells is regulated both at the transcriptional and translational level",

abstract = "The T-cell specific adapter protein (TSAd) encoded by the SH2D2A gene is up-regulated in activated human CD4+ T-cells in a cAMP-dependent manner. Expression of SH2D2A is important for proper activation of T-cells. Here, we show that SH2D2A expression is regulated both at the transcriptional and translational level. cAMP signaling alone induces TSAd-mRNA expression but fails to induce increased TSAd protein levels. By contrast, TCR engagement provides signals for both TSAd transcription and translation. We further show that cAMP signaling can prime T-cells for a more prompt expression of TSAd protein upon TCR stimulation. Our study thus points to a novel mechanism for how cAMP signaling may modulate T-cell activation through transcriptional priming of resting cells.",

keywords = "Adapter protein, Gene expression regulation, T-cell activation, cAMP",

author = "Kolltveit, {Kristin Melkevik} and Stine Granum and Aasheim, {Hans Christian} and Monika Forsbring and Vibeke Sundvold-Gjerstad and Dai, {Ke Zheng} and {\O}yvind Molberg and Schjetne, {Karoline W.} and Bjarne Bogen and Shapiro, {Virginia S.} and Johansen, {Finn Eirik} and Karl Schenck and Anne Spurkland",

note = "Funding Information: The technical assistance of Hege B. Gehrken and Hanne Weidemann is highly appreciated. Tone Berge is thanked for critically reading the manuscript. This study was supported by grants from the Norwegian Research Council, Faculty of Odontology, University of Oslo, the Norwegian Cancer Society, Novo Nordisk Fonden, Unit for molecular biology (EMBIO) at the University of Oslo, and Anders Jahre research fund.",

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doi = "10.1016/j.molimm.2007.11.005",

language = "English (US)",

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pages = "2380--2390",

journal = "Molecular Immunology",

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AU - Kolltveit, Kristin Melkevik

AU - Granum, Stine

AU - Aasheim, Hans Christian

AU - Forsbring, Monika

AU - Sundvold-Gjerstad, Vibeke

AU - Dai, Ke Zheng

AU - Molberg, Øyvind

AU - Schjetne, Karoline W.

AU - Bogen, Bjarne

AU - Shapiro, Virginia S.

AU - Johansen, Finn Eirik

AU - Schenck, Karl

AU - Spurkland, Anne

N1 - Funding Information: The technical assistance of Hege B. Gehrken and Hanne Weidemann is highly appreciated. Tone Berge is thanked for critically reading the manuscript. This study was supported by grants from the Norwegian Research Council, Faculty of Odontology, University of Oslo, the Norwegian Cancer Society, Novo Nordisk Fonden, Unit for molecular biology (EMBIO) at the University of Oslo, and Anders Jahre research fund.

PY - 2008/4

Y1 - 2008/4

N2 - The T-cell specific adapter protein (TSAd) encoded by the SH2D2A gene is up-regulated in activated human CD4+ T-cells in a cAMP-dependent manner. Expression of SH2D2A is important for proper activation of T-cells. Here, we show that SH2D2A expression is regulated both at the transcriptional and translational level. cAMP signaling alone induces TSAd-mRNA expression but fails to induce increased TSAd protein levels. By contrast, TCR engagement provides signals for both TSAd transcription and translation. We further show that cAMP signaling can prime T-cells for a more prompt expression of TSAd protein upon TCR stimulation. Our study thus points to a novel mechanism for how cAMP signaling may modulate T-cell activation through transcriptional priming of resting cells.

AB - The T-cell specific adapter protein (TSAd) encoded by the SH2D2A gene is up-regulated in activated human CD4+ T-cells in a cAMP-dependent manner. Expression of SH2D2A is important for proper activation of T-cells. Here, we show that SH2D2A expression is regulated both at the transcriptional and translational level. cAMP signaling alone induces TSAd-mRNA expression but fails to induce increased TSAd protein levels. By contrast, TCR engagement provides signals for both TSAd transcription and translation. We further show that cAMP signaling can prime T-cells for a more prompt expression of TSAd protein upon TCR stimulation. Our study thus points to a novel mechanism for how cAMP signaling may modulate T-cell activation through transcriptional priming of resting cells.

KW - Adapter protein

KW - Gene expression regulation

KW - T-cell activation

KW - cAMP

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Expression of SH2D2A in T-cells is regulated both at the transcriptional and translational level

Abstract

Keywords

ASJC Scopus subject areas

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