TY - GEN
T1 - Experimental validation of an optofluidic platform for microbial single cell isolation and whole genome amplification for human microbiome applications
AU - Liu, Yuguang
AU - Jeraldo, Patricio
AU - McDonough, Samantha
AU - Jen, Jin
AU - Patel, Robin
AU - Walther-Antonio, Marina
AU - Lambert, Christopher
AU - Gale, Bruce
N1 - Funding Information:
ACKNOWLEDGMENT This work is philanthropically funded by the Ivan Bowen Family Foundation. The authors gratefully acknowlege individuals for their support in this study. Paul Blainey advised on the re-construction of the platform at Mayo Clinic. Zachary Landry provided valuable experience on the on-chip SC-WGA. The Mayo Clinic Genome Analysis Core provided technical support and sequencing of the samples involved in this study. Janet Yao provided much assistance during the testing rpocess.
Publisher Copyright:
© 2017 IEEE.
PY - 2017/7/19
Y1 - 2017/7/19
N2 - Single microbial cell genome sequencing is becoming a powerful tool for the discovery of the hidden genetic information valuable for many medical applications. One of the critical steps in single-cell genome sequencing is the physical isolation of individual cells from a highly diverse heterogeneous population. Amplifying the genome of a single microbial cell is another challenge due to the minute amount of DNA. Efforts have been directed in developing an optofluidic platform integrating advanced microscopy, optical tweezers and microfluidic technology for single cell isolation and genome amplification. Here, we investigate and evaluate the validity of this platform for single microbial cell genome amplification. The successful validation of this approach allows us to perform various single cell studies using this platform.
AB - Single microbial cell genome sequencing is becoming a powerful tool for the discovery of the hidden genetic information valuable for many medical applications. One of the critical steps in single-cell genome sequencing is the physical isolation of individual cells from a highly diverse heterogeneous population. Amplifying the genome of a single microbial cell is another challenge due to the minute amount of DNA. Efforts have been directed in developing an optofluidic platform integrating advanced microscopy, optical tweezers and microfluidic technology for single cell isolation and genome amplification. Here, we investigate and evaluate the validity of this platform for single microbial cell genome amplification. The successful validation of this approach allows us to perform various single cell studies using this platform.
KW - microbiome
KW - microfluidics
KW - optical tweezers
KW - optofluidics
KW - single cell
KW - whole genome amplification
UR - http://www.scopus.com/inward/record.url?scp=85027882234&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85027882234&partnerID=8YFLogxK
U2 - 10.1109/MeMeA.2017.7985850
DO - 10.1109/MeMeA.2017.7985850
M3 - Conference contribution
AN - SCOPUS:85027882234
T3 - 2017 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2017 - Proceedings
SP - 62
EP - 66
BT - 2017 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2017 - Proceedings
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 12th IEEE International Symposium on Medical Measurements and Applications, MeMeA 2017
Y2 - 7 May 2017 through 10 May 2017
ER -