Experimental model of aortic valve calcification to induce osteoblast differentiation

Nalini M. Rajamannan, Muzaffer Cicek, John R. Hawse, Thomas C. Spelsberg, Malayannan Subramaniam

Research output: Chapter in Book/Report/Conference proceedingChapter


Over the past 25 years, the development of an in vivo model to test for calcific aortic valve disease has been challenging. The understanding of the biology, imaging, and duration of exposure to risk factors, have been the cornerstone for defining the different stages of osteoblast differentiation. The first experimental was high cholesterol-diet rabbit model. The studies published demonstrated apoptosis [1], cell proliferation [2], and atherosclerosis [1, 3-6] along the aortic valve surface. These models all include short time diet experiments to define the early atherosclerotic findings in the valve. The next level of experimentation includes testing the diet for 6 months. This duration of diet provides the time necessary for the valve to mineralize and to calcify.

Original languageEnglish (US)
Title of host publicationMolecular Biology of Valvular Heart Disease
PublisherSpringer-Verlag London Ltd
Number of pages7
ISBN (Electronic)9781447163503
ISBN (Print)9781447163497
StatePublished - Jan 1 2014

ASJC Scopus subject areas

  • Medicine(all)


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