Exome Sequencing of Pancreatic Acinar Carcinoma Identified Distinctive Mutation Patterns

Yuanhang Liu, Massimo Raimondo, Michael B. Wallace, Kabir Mody, John A. Stauffer, Lizhi Zhang, Baoan Ji, Yan Bi

Research output: Contribution to journalArticlepeer-review


Objectives Pancreatic acinar cell carcinoma (ACC) is a rare pancreatic cancer. The advancement of treatment is hampered because of the limited knowledge of its molecular mechanism. Methods Whole-exome sequencing was performed on DNA extracted from 11 pure ACC surgical samples. Potential germline variants were removed on the basis of polymorphic databases, alternative allele frequency, coverage depth, and Catalogue of Somatic Mutations in Cancer (COSMIC) annotations after variant calling procedure. Mutation profiles and signatures were assessed through the Mutational Patterns package. Results A median of 34 somatic mutations were detected (range, 19-60). Three novel recurrent small deletions were identified. Common pancreatic ductal adenocarcinoma mutations or neuroendocrine tumor mutants were not found. FAT atypical cadherin 4, mucin 5B, titin, and zinc finger homeobox 3 were consistently mutated across 4 independent ACC studies. A high contribution of COSMIC mutational signature 1 was seen in ACC, indicating deamination of 5-methylcytosine. The majority of the patients had COSMIC signatures 6, 15, or 20, relating to defective DNA mismatch repair. Six patients showed COSMIC mutational signature 10 because of the altered activity of DNA polymerase epsilon. Conclusions Distinct mutational signatures pathways were found in ACC and targeting them may improve clinical outcome.

Original languageEnglish (US)
Pages (from-to)1007-1013
Number of pages7
Issue number7
StatePublished - Aug 1 2021


  • acinar cell carcinoma
  • exome sequencing
  • mutation

ASJC Scopus subject areas

  • Endocrinology
  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology


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