Evaluation of Pericardial Tissues from Assorted Species as a Tissue-Engineered Heart Valve Material

Christopher Noble, David Morse, Amir Lerman, Melissa Young

Research output: Contribution to journalArticlepeer-review


Decellularized pericardial tissue is a strong candidate for a TEHV material as ECM is present to guide cellular infiltration and fixed porcine and bovine pericardial tissue have existing use in bioprosthetic heart valves. In this work, we compare the mechanical and microstructural properties of decellularized-sterilized (DS) porcine, bovine, and bison pericardial tissues with respect to use as a TEHV. H&E staining was used to verify removal of cellular content post-decellularization and to evaluate collagen fiber structure. Additionally, uniaxial and biaxial tension testing were used to compare mechanical performance and, for the latter, acquire constitutive model parameters for subsequent finite element (FE) modeling. H&E staining revealed complete removal of cellular content and good collagen fiber structure. Tensile testing showed comparable mechanical strength between the three DS pericardial tissues and considerably stronger mechanical properties compared to native tissues. Bovine and bison DS pericardial tissues showed the strongest mechanical performance in the FE models with bison demonstrating the overall best mechanical characteristics. The increased thickness of bovine and bison tissues coupled with the strong mechanical behavior and ECM structure indicates that these materials will be resistant to damage until sufficient cellular infiltration has occurred such that damaged tissue can be repaired. Graphical abstract: [Figure not available: see fulltext.]

Original languageEnglish (US)
Pages (from-to)393-406
Number of pages14
JournalMedical and Biological Engineering and Computing
Issue number2
StatePublished - Feb 2022


  • Bison pericardium
  • Decellularized pericardium
  • Finite element analysis
  • Tensile testing
  • Tissue-engineered heart valve

ASJC Scopus subject areas

  • Biomedical Engineering
  • Computer Science Applications


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