TY - JOUR
T1 - Estrogen influences the differentiation, proliferation, and survival of early B-lineage precursors
AU - Medina, Kay L.
AU - Strasser, Andreas
AU - Kincade, Paul W.
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2000/3/15
Y1 - 2000/3/15
N2 - B lymphocyte production in murine bone marrow is negatively regulated by sex steroids and the aim of this study was to identify early hormone sensitive checkpoints. Estrogen (E2) treatment reduced cμ+ pre-B cells, a change that occurred concomitantly with decreased Ig gene rearrangements and rag-1 transcripts. Estrogen decreased B lineage precursors in Ig transgenic mice, demonstrating that resistant to estrogen treatment, suggestIng that life/death decisions are involved in hormonal regulation. A previously uncharacterized population of CD43-cμ- B lineage precursors was identified in normal, Ig transgenic, and RAG(-/-) mice after estrogen treatment, revealing that down-regulation of CD43 can occur independent of Ig heavy chain expression. These cells expressed transcripts for both tdt and bcl-2, characteristics of early B-cell precursors. BrdU incorporation analysis revealed that the mitotic activity of early B-lineage cells is reduced in hormone-treated mice. We conclude that sex steroids modulate the production of B-lineage cells by influencing the differentiation, proliferation, and survival of early B-cell precursors. These findings are informative about mechanisms of hormonal regulation, as well as the significance of some differentiation-related events. (C) 2000 by The American Society of Hematology.
AB - B lymphocyte production in murine bone marrow is negatively regulated by sex steroids and the aim of this study was to identify early hormone sensitive checkpoints. Estrogen (E2) treatment reduced cμ+ pre-B cells, a change that occurred concomitantly with decreased Ig gene rearrangements and rag-1 transcripts. Estrogen decreased B lineage precursors in Ig transgenic mice, demonstrating that resistant to estrogen treatment, suggestIng that life/death decisions are involved in hormonal regulation. A previously uncharacterized population of CD43-cμ- B lineage precursors was identified in normal, Ig transgenic, and RAG(-/-) mice after estrogen treatment, revealing that down-regulation of CD43 can occur independent of Ig heavy chain expression. These cells expressed transcripts for both tdt and bcl-2, characteristics of early B-cell precursors. BrdU incorporation analysis revealed that the mitotic activity of early B-lineage cells is reduced in hormone-treated mice. We conclude that sex steroids modulate the production of B-lineage cells by influencing the differentiation, proliferation, and survival of early B-cell precursors. These findings are informative about mechanisms of hormonal regulation, as well as the significance of some differentiation-related events. (C) 2000 by The American Society of Hematology.
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U2 - 10.1182/blood.v95.6.2059
DO - 10.1182/blood.v95.6.2059
M3 - Article
C2 - 10706875
AN - SCOPUS:0034654329
SN - 0006-4971
VL - 95
SP - 2059
EP - 2067
JO - Blood
JF - Blood
IS - 6
ER -