TY - JOUR
T1 - Estrogen and progesterone receptors in meningiomas
T2 - Comparison of nuclear binding, dextran-coated charcoal, and immunoperoxidase staining assays
AU - Halper, J.
AU - Colvard, D. S.
AU - Scheithauer, B. W.
AU - Jiang, N. S.
AU - Press, M. F.
AU - Graham, M. L.
AU - Riehl, E.
AU - Laws, E. R.
AU - Spelsberg, T. C.
PY - 1989
Y1 - 1989
N2 - We studied the status of estrogen (ER) and progesterone (PR) receptors in meningiomas removed from 52 patients, comparing dextran-coated charcoal (DCC), nuclear binding (NB), and immunoperoxidase (IP) assays. Each of the assays was performed indepdendently by investigators well-experienced with these assays. The NB assay is a new assay that measures functional steroid receptors - that is, the activation of the receptor and its binding to the nucleus. The assay is very sensitive and requires a relatively small amount of tissue as compared with the DCC assay. In agreement with data from other studies, PR were detected in most meningiomas by all 3 methods: in 69% of the cases by NB, in 76% by DCC, and in 89% by IP. ER were detected in only a few cases: in 33% by NB, in 2% by DCC, and in none by the IP assay. The aagreement for PR sites was 62% for all 3 assays; it was 66% between the NB and DCC assays, 67% bertween the NB and IP assays, and 86% between the DCC and IP assays. Of 26 cases that were positive by the DCC assay, 6 (23%) were negative by NB. The overall agreement for all three ER assays was 65%. The data suggest that the majority of meningiomas contain high-affinity receptors for progesterone, that estrogen receptors are present in only a few meningiomas, and that some of these estrogen and progesterone receptors appear to be function.
AB - We studied the status of estrogen (ER) and progesterone (PR) receptors in meningiomas removed from 52 patients, comparing dextran-coated charcoal (DCC), nuclear binding (NB), and immunoperoxidase (IP) assays. Each of the assays was performed indepdendently by investigators well-experienced with these assays. The NB assay is a new assay that measures functional steroid receptors - that is, the activation of the receptor and its binding to the nucleus. The assay is very sensitive and requires a relatively small amount of tissue as compared with the DCC assay. In agreement with data from other studies, PR were detected in most meningiomas by all 3 methods: in 69% of the cases by NB, in 76% by DCC, and in 89% by IP. ER were detected in only a few cases: in 33% by NB, in 2% by DCC, and in none by the IP assay. The aagreement for PR sites was 62% for all 3 assays; it was 66% between the NB and DCC assays, 67% bertween the NB and IP assays, and 86% between the DCC and IP assays. Of 26 cases that were positive by the DCC assay, 6 (23%) were negative by NB. The overall agreement for all three ER assays was 65%. The data suggest that the majority of meningiomas contain high-affinity receptors for progesterone, that estrogen receptors are present in only a few meningiomas, and that some of these estrogen and progesterone receptors appear to be function.
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U2 - 10.1227/00006123-198910000-00007
DO - 10.1227/00006123-198910000-00007
M3 - Article
C2 - 2477767
AN - SCOPUS:0024401650
SN - 0148-396X
VL - 25
SP - 546
EP - 553
JO - Neurosurgery
JF - Neurosurgery
IS - 4
ER -