ESCRT ubiquitin-binding domains function cooperatively during MVB cargo sorting

S. Brookhart Shields, Andrea J. Oestreich, Stanley Winistorfer, Doris Nguyen, Johanna A. Payne, David J. Katzmann, Robert Piper

Research output: Contribution to journalArticlepeer-review

74 Scopus citations


Ubiquitin (Ub) sorting receptors facilitate the targeting of ubiquitinated membrane proteins into multivesicular bodies (MVBs). Ub-binding domains (UBDs) have been described in several endosomal sorting complexes required for transport (ESCRT). Using available structural information, we have investigated the role of the multiple UBDs within ESCRTs during MVB cargo selection. We found a novel UBD within ESCRT-I and show that it contributes to MVB sorting in concert with the known UBDs within the ESCRT complexes. These experiments reveal an unexpected level of coordination among the ESCRT UBDs, suggesting that they collectively recognize a diverse set of cargo rather than act sequentially at discrete steps.

Original languageEnglish (US)
Pages (from-to)213-224
Number of pages12
JournalJournal of Cell Biology
Issue number2
StatePublished - Apr 20 2009

ASJC Scopus subject areas

  • Cell Biology


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