TY - JOUR
T1 - ESCRT ubiquitin-binding domains function cooperatively during MVB cargo sorting
AU - Shields, S. Brookhart
AU - Oestreich, Andrea J.
AU - Winistorfer, Stanley
AU - Nguyen, Doris
AU - Payne, Johanna A.
AU - Katzmann, David J.
AU - Piper, Robert
PY - 2009/4/20
Y1 - 2009/4/20
N2 - Ubiquitin (Ub) sorting receptors facilitate the targeting of ubiquitinated membrane proteins into multivesicular bodies (MVBs). Ub-binding domains (UBDs) have been described in several endosomal sorting complexes required for transport (ESCRT). Using available structural information, we have investigated the role of the multiple UBDs within ESCRTs during MVB cargo selection. We found a novel UBD within ESCRT-I and show that it contributes to MVB sorting in concert with the known UBDs within the ESCRT complexes. These experiments reveal an unexpected level of coordination among the ESCRT UBDs, suggesting that they collectively recognize a diverse set of cargo rather than act sequentially at discrete steps.
AB - Ubiquitin (Ub) sorting receptors facilitate the targeting of ubiquitinated membrane proteins into multivesicular bodies (MVBs). Ub-binding domains (UBDs) have been described in several endosomal sorting complexes required for transport (ESCRT). Using available structural information, we have investigated the role of the multiple UBDs within ESCRTs during MVB cargo selection. We found a novel UBD within ESCRT-I and show that it contributes to MVB sorting in concert with the known UBDs within the ESCRT complexes. These experiments reveal an unexpected level of coordination among the ESCRT UBDs, suggesting that they collectively recognize a diverse set of cargo rather than act sequentially at discrete steps.
UR - http://www.scopus.com/inward/record.url?scp=65349101661&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65349101661&partnerID=8YFLogxK
U2 - 10.1083/jcb.200811130
DO - 10.1083/jcb.200811130
M3 - Article
C2 - 19380877
AN - SCOPUS:65349101661
SN - 0021-9525
VL - 185
SP - 213
EP - 224
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 2
ER -