Eosinophil granule proteins in peripheral blood granulocytes

R. I. Abu-Ghazaleh, S. L. Dunnette, D. A. Loegering, J. L. Checkel, H. Kita, L. L. Thomas, G. J. Gleich

Research output: Contribution to journalArticlepeer-review

154 Scopus citations


Eosinophils contain four principal cationic proteins, major basic protein (MBP), eosinophil-derived neurotoxin (EDN), eosinophil cationic protein (ECP), and eosinophil peroxidase (EPO). To determine the quantities of these proteins in granulocytes and whether they are specific to eosinophils, their concentrations in lysates of human granulocytes were measured using specific radioimmunoassays. The effect of different methods for eosinophil lysis on the recovery of the proteins was also studied. Maximal recovery occurred at pH 2 for MBP and pH 5.6 for the other granule proteins. The proteins cosedimented with eosinophils and their concentrations (X̄ ± SEM) in ng/106 eosinophils (and in nM/106 eosinophils) were: MBP, 8,982 ± 611 (641.6); EDN, 3,283 ± 116 (178.4); ECP, 5,269 ± 283 (250.9); and EPO, 12,174 ± 859 (171.5). Basophils from a normal person contained (in ng/106 cells) MBP, 2,374; EDN, 214; ECP, 77; and EPO, 17. Highly purified neutrophils contained (in ng/106 cells) MBP, 3 ± 0.5; EDN, 72 ± 9; and ECP, 50 ± 12. Therefore we conclude that these proteins are mainly expressed in eosinophils, but that certain ones are present in basophils and neutrophils.

Original languageEnglish (US)
Pages (from-to)611-618
Number of pages8
JournalJournal of Leukocyte Biology
Issue number6
StatePublished - 1992


  • basophils
  • cationic protein
  • eosinophil
  • eosinophil peroxidase
  • eosinophil-derived neurotoxin
  • major basic protein
  • neutrophils

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology


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