TY - JOUR
T1 - Enzymatic-synthesis and degradation of nicotinate adenine dinucleotide phosphate (NAADP), a Ca2+-releasing agonist, in rat tissues
AU - Chini, Eduardo N.
AU - Dousa, Thomas P.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1995/4/6
Y1 - 1995/4/6
N2 - We have recently found that nicotinate adenine dinucleotide phosphate (NAADP) is a potent agonist that triggers Ca2+ release from intracellular stores of sea urchin eggs, and that its action is distinct from effects of IP3 and cyclic ADP-ribose (J. Biol. Chem. 270:3216, 1995). Now we report: that extracts from rat brain, heart, liver, and spleen but not kidney cortex contain enzymatic activity which catalyzes NAADP synthesis by exchange of nicotinamide for nicotinic acid and which is probably catalyzed by NAD(P)-glycohydrolase. Extracts from these tissues also inactivate NAADP in the rank inverse to their ability for NAADP synthesis. These results suggest that NAADP, a Ca2+ -releasing agent, can be generated in mammalian tissues, namely in brain.
AB - We have recently found that nicotinate adenine dinucleotide phosphate (NAADP) is a potent agonist that triggers Ca2+ release from intracellular stores of sea urchin eggs, and that its action is distinct from effects of IP3 and cyclic ADP-ribose (J. Biol. Chem. 270:3216, 1995). Now we report: that extracts from rat brain, heart, liver, and spleen but not kidney cortex contain enzymatic activity which catalyzes NAADP synthesis by exchange of nicotinamide for nicotinic acid and which is probably catalyzed by NAD(P)-glycohydrolase. Extracts from these tissues also inactivate NAADP in the rank inverse to their ability for NAADP synthesis. These results suggest that NAADP, a Ca2+ -releasing agent, can be generated in mammalian tissues, namely in brain.
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U2 - 10.1006/bbrc.1995.1485
DO - 10.1006/bbrc.1995.1485
M3 - Article
C2 - 7726831
AN - SCOPUS:0028963107
SN - 0006-291X
VL - 209
SP - 167
EP - 174
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -