TY - JOUR
T1 - Enzymatic determination of total 3αhydroxy bile acids in faeces
T2 - Validation in healthy subjects of a rapid method suitable for clinical routine purpose
AU - Malchow-Møller, A.
AU - Arffmann, S.
AU - Larusso, N. F.
AU - Krag, E.
N1 - Funding Information:
This study was supported by The Danish Medical Research Council, grant 512-16144. Mrs. Kirsten Rasmussen rendered expert assistance.
PY - 1982
Y1 - 1982
N2 - A method for determining faecal bile acids, suitable for clinical purposes, is introduced. The analysis uses a 0.2-g stool specimen, a simple extraction procedure, and 3αsteroid dehydrogenase determination. The method, which is rapid, has been validated by gas-liquid chromatography and by recovery of internal standards. Stool examination was done in 16 healthy volunteers on free diet and in 25 patients with non-gastrointestinal diseases who were on a fat- and fibre-fixed diet. No difference was found between the two groups, so the data were pooled, and the normal reference interval (X ± 2S.D.) for faecal bile acid output was calculated to be 0-975 μmol/24h.
AB - A method for determining faecal bile acids, suitable for clinical purposes, is introduced. The analysis uses a 0.2-g stool specimen, a simple extraction procedure, and 3αsteroid dehydrogenase determination. The method, which is rapid, has been validated by gas-liquid chromatography and by recovery of internal standards. Stool examination was done in 16 healthy volunteers on free diet and in 25 patients with non-gastrointestinal diseases who were on a fat- and fibre-fixed diet. No difference was found between the two groups, so the data were pooled, and the normal reference interval (X ± 2S.D.) for faecal bile acid output was calculated to be 0-975 μmol/24h.
KW - Bile acids
KW - Faeces
UR - http://www.scopus.com/inward/record.url?scp=0020051586&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020051586&partnerID=8YFLogxK
U2 - 10.3109/00365528209182063
DO - 10.3109/00365528209182063
M3 - Article
C2 - 6957975
AN - SCOPUS:0020051586
SN - 0036-5521
VL - 17
SP - 331
EP - 333
JO - Scandinavian Journal of Gastroenterology
JF - Scandinavian Journal of Gastroenterology
IS - 3
ER -