Enthesis Healing Is Dependent on Scaffold Interphase Morphology—Results from a Rodent Patellar Model

Carlos J. Peniche Silva, Sebastian A. Müller, Nicholas Quirk, Patrina S.P. Poh, Carla Mayer, Antonella Motta, Claudio Migliaresi, Michael J. Coenen, Christopher H. Evans, Elizabeth R. Balmayor, Martijn van Griensven

Research output: Contribution to journalArticlepeer-review


The use of multiphasic scaffolds to treat injured tendon-to-bone entheses has shown promising results in vitro. Here, we used two versions of a biphasic silk fibroin scaffold to treat an enthesis defect created in a rat patellar model in vivo. One version presented a mixed transition between the bony and the tendon end of the construct (S-MT) while this transition was abrupt in the second version (S-AT). At 12 weeks after surgery, the S-MT scaffold promoted better healing of the injured enthesis, with minimal undesired ossification of the insertion area. The expression of tenogenic and chondrogenic markers was sustained for longer in the S-MT-treated group and the tangent modulus of the S-MT-treated samples was similar to the native tissue at 12 weeks while that of the S-AT-treated enthesis was lower. Our study highlights the important role of the transition zone of multiphasic scaffolds in the treatment of complex interphase tissues such as the tendon-to-bone enthesis.

Original languageEnglish (US)
Article number1752
Issue number11
StatePublished - Jun 1 2022


  • enthesis
  • multiphasic
  • scaffold
  • silk fibroin
  • tendon

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


Dive into the research topics of 'Enthesis Healing Is Dependent on Scaffold Interphase Morphology—Results from a Rodent Patellar Model'. Together they form a unique fingerprint.

Cite this