Engineered tendon with decellularized xenotendon slices and bone marrow stromal cells: An in vivo animal study

Hiromichi Omae, Yu Long Sun, Kai Nan An, Peter C. Amadio, Chunfeng Zhao

Research output: Contribution to journalArticlepeer-review

47 Scopus citations


The purpose of this study was to investigate an engineered composite of multilayer acellular tendon slices seeded with bone marrow stromal cells (BMSCs) as a possible solution for tendon reconstruction. BMSCs were harvested from 15 rabbits and infraspinatus tendons were harvested from 17 dogs. The decellularized tendons were sectioned in longitudinal slices with a thickness of 50 μm. The BMSCs were seeded on the slices and then the slices were bundled into one composite. The composite was implanted into a rabbit patellar tendon defect. Tendon slices without BMSCs were implanted into the contralateral patellar tendon as a control. The composites were evaluated by histology and qRT-PCR. The viability of BMSCs was assessed using a fluorescent marker. Histology showed viable cells between the collagen fibres on the cell-seeded side. Analysis by qRT-PCR showed higher tenomodulin, collagen type III, MMP3 and MMP13 expressions and lower collagen type I expression in the cell-seeded composite than in the tendon slices without BMSCs. We conclude that BMSCs can survive in a multilayer composite, express a tendon phenotype and enhance the metabolism of tendon in vivo. This in vivo study suggests a potential utility of this composite in tendon reconstruction.

Original languageEnglish (US)
Pages (from-to)238-244
Number of pages7
JournalJournal of Tissue Engineering and Regenerative Medicine
Issue number3
StatePublished - Mar 2012


  • Bone marrow stromal cells
  • Cell tracking
  • Gene expression
  • Regeneration
  • Tendon
  • Tissue engineering

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering


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