Eliminating false-positive results in serum tests for neuromuscular autoimmunity

Metha Apiwattanakul; Andrew McKeon; Sean J. Pittock; Thomas J. Kryzer; Vanda A. Lennon

doi:10.1002/mus.21653

Eliminating false-positive results in serum tests for neuromuscular autoimmunity

Metha Apiwattanakul, Andrew McKeon, Sean J. Pittock, Thomas J. Kryzer, Vanda A. Lennon

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

In this study we describe the false-positive frequency in radioimmunoprecipitation assays for muscle acetylcholine receptor (AChR) and neuronal voltage-gated potassium channel (VGKC) autoantibodies, attributable to ¹²⁵I-ligand immunoprecipitation. Sera were evaluated for AChR autoantibody (n = 34,095) and VGKC autoantibody (n = 11,028). We retested sera that yielded apparently positive results with ¹²⁵1-ligand with and without detergent-solubilized cation-channel protein and indentified clinically validated fals-positive rates of 0.05% and 1.7% for AchR and VGKC autoantibodies, respectively. Specificity assurance in radioimmunoprecipitation assays requires subtraction of values for ¹²⁵I-ligand binding.

Original language	English (US)
Pages (from-to)	702-704
Number of pages	3
Journal	Muscle and Nerve
Volume	41
Issue number	5
DOIs	https://doi.org/10.1002/mus.21653
State	Published - May 1 2010

Keywords

Acetylcholine receptor autoantibody
Myasthenia gravis
Potassium channel autoantibody
α-bungarotoxin
α-dendrotoxin

ASJC Scopus subject areas

Physiology
Clinical Neurology
Cellular and Molecular Neuroscience
Physiology (medical)

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10.1002/mus.21653

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abstract = "In this study we describe the false-positive frequency in radioimmunoprecipitation assays for muscle acetylcholine receptor (AChR) and neuronal voltage-gated potassium channel (VGKC) autoantibodies, attributable to 125I-ligand immunoprecipitation. Sera were evaluated for AChR autoantibody (n = 34,095) and VGKC autoantibody (n = 11,028). We retested sera that yielded apparently positive results with 1251-ligand with and without detergent-solubilized cation-channel protein and indentified clinically validated fals-positive rates of 0.05% and 1.7% for AchR and VGKC autoantibodies, respectively. Specificity assurance in radioimmunoprecipitation assays requires subtraction of values for 125I-ligand binding.",

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author = "Metha Apiwattanakul and Andrew McKeon and Pittock, {Sean J.} and Kryzer, {Thomas J.} and Lennon, {Vanda A.}",

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AU - McKeon, Andrew

AU - Pittock, Sean J.

AU - Kryzer, Thomas J.

AU - Lennon, Vanda A.

PY - 2010/5/1

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KW - α-bungarotoxin

KW - α-dendrotoxin

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Eliminating false-positive results in serum tests for neuromuscular autoimmunity

Abstract

Keywords

ASJC Scopus subject areas

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