Elevated levels of phosphorylated fibrinogen-α-isoforms and differential expression of other post-translationally modified proteins in the plasma of ovarian cancer patients

Yuko Ogata; Carrie J. Hepplmann; M. Cristine Charlesworth; Benjamin J. Madden; Melinda N. Miller; Kimberly R. Kalli; William A. Cilby; H. Robert Bergen; Diana A. Saggese; David G. Muddiman

doi:10.1021/pr060344+

Elevated levels of phosphorylated fibrinogen-α-isoforms and differential expression of other post-translationally modified proteins in the plasma of ovarian cancer patients

Yuko Ogata, Carrie J. Hepplmann, M. Cristine Charlesworth, Benjamin J. Madden, Melinda N. Miller, Kimberly R. Kalli, William A. Cilby, H. Robert Bergen, Diana A. Saggese, David G. Muddiman

Biochemistry and Molecular Biology

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45 Scopus citations

Abstract

We evaluated the differentially expressed proteins in the plasma of ovarian cancer (OVC) patients using 2-D SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with post-translational modification (PTM) specific stains after the removal of six high-abundance proteins. The pooled plasma from patients with stage III or IV OVC was compared to a pooled postmenopausal age-matched control. Several proteins were identified as differentially expressed in the plasma of OVC patients. Among them, the phosphorylated fibrinogen-α-chain isoform (containing fibrinopeptide-A) was found to be up-regulated. Previously in our laboratory, phosphorylated fibrinopeptide-A was found to be up-regulated in the low molecular weight fraction of serum derived from OVC patients. We examined the levels of phosphorylated fibrinogen-α-chain in each patient that constituted the pooled plasma using Western blot, mass spectrometry (MS), and PTM specific stains. Phosphoprotein bands containing fibrinogen-α-chain fragments showed up-regulation in all OVC patients.

Original language	English (US)
Pages (from-to)	3318-3325
Number of pages	8
Journal	Journal of Proteome Research
Volume	5
Issue number	12
DOIs	https://doi.org/10.1021/pr060344+
State	Published - Dec 2006

Keywords

Biomarker
Fibrinogen
Glycosylation
Mass spectrometry
Ovarian cancer
Phosphorylation
Protein depletion
Two-dimensional gel electrophoresis
Western blot

ASJC Scopus subject areas

Biochemistry
Chemistry(all)

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10.1021/pr060344+

Cite this

Ogata, Y., Hepplmann, C. J., Charlesworth, M. C., Madden, B. J., Miller, M. N., Kalli, K. R., Cilby, W. A., Bergen, H. R., Saggese, D. A., & Muddiman, D. G. (2006). Elevated levels of phosphorylated fibrinogen-α-isoforms and differential expression of other post-translationally modified proteins in the plasma of ovarian cancer patients. Journal of Proteome Research, 5(12), 3318-3325. https://doi.org/10.1021/pr060344+

Elevated levels of phosphorylated fibrinogen-α-isoforms and differential expression of other post-translationally modified proteins in the plasma of ovarian cancer patients. / Ogata, Yuko; Hepplmann, Carrie J.; Charlesworth, M. Cristine et al.
In: Journal of Proteome Research, Vol. 5, No. 12, 12.2006, p. 3318-3325.

Research output: Contribution to journal › Article › peer-review

Ogata, Y, Hepplmann, CJ, Charlesworth, MC, Madden, BJ, Miller, MN, Kalli, KR, Cilby, WA, Bergen, HR, Saggese, DA & Muddiman, DG 2006, 'Elevated levels of phosphorylated fibrinogen-α-isoforms and differential expression of other post-translationally modified proteins in the plasma of ovarian cancer patients', Journal of Proteome Research, vol. 5, no. 12, pp. 3318-3325. https://doi.org/10.1021/pr060344+

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abstract = "We evaluated the differentially expressed proteins in the plasma of ovarian cancer (OVC) patients using 2-D SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with post-translational modification (PTM) specific stains after the removal of six high-abundance proteins. The pooled plasma from patients with stage III or IV OVC was compared to a pooled postmenopausal age-matched control. Several proteins were identified as differentially expressed in the plasma of OVC patients. Among them, the phosphorylated fibrinogen-α-chain isoform (containing fibrinopeptide-A) was found to be up-regulated. Previously in our laboratory, phosphorylated fibrinopeptide-A was found to be up-regulated in the low molecular weight fraction of serum derived from OVC patients. We examined the levels of phosphorylated fibrinogen-α-chain in each patient that constituted the pooled plasma using Western blot, mass spectrometry (MS), and PTM specific stains. Phosphoprotein bands containing fibrinogen-α-chain fragments showed up-regulation in all OVC patients.",

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AU - Kalli, Kimberly R.

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AU - Saggese, Diana A.

AU - Muddiman, David G.

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Elevated levels of phosphorylated fibrinogen-α-isoforms and differential expression of other post-translationally modified proteins in the plasma of ovarian cancer patients

Abstract

Keywords

ASJC Scopus subject areas

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