Elevated levels of M(r) 92,000 type IV collagenase during tumor growth in vivo

Raymond Sawaya, Yoshinori Go, Athanassios P. Kyritisis, Joon Uhm, Boyapati Venkaiah, Sanjeeva Mohanam, Ziya L. Gokaslan, Jasti S. Rao

Research output: Contribution to journalArticlepeer-review

46 Scopus citations


Our previous studies demonstrated that matrix metalloproteinase (MMP-9) levels were significantly higher in human glioblastoma tissue samples than in low-grade brain tumors and normal brain tissue. In the present study, we measured the levels of MMP-2 and MMP-9 during the growth of glial tumors in nude mice by intracerebral injection of glioblastoma cells. Using gelatin zymography, densitometry, and an enzyme-linked immunosorbent assay, we found that the enzyme activity and protein count of MMP-2 and MMP-9 were a respective 3- to 10- and 2- to 30-fold higher in tumors at day 14 and 28 than in normal tissue. Immunohistochemical staining for MMP-9 showed strong immunoreactivity in tumor cells and the staining intensity was much higher at day 28, compared to day 14. These results suggest that upregulation of MMP-9 plays a major role in the glioma tumor growth in vivo.

Original languageEnglish (US)
Pages (from-to)632-636
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - Oct 20 1998

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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