Electroejaculation of chimeric rats

Marina R. Mccoy; Daniel Montonye; Elizabeth C. Bryda

doi:10.1038/laban.301

Electroejaculation of chimeric rats

Marina R. Mccoy, Daniel Montonye, Elizabeth C. Bryda

Comparative Medicine (Veterinary Medicine)

Research output: Contribution to journal › Article › peer-review

Abstract

With the advent of genetic engineering of rodents came the need to assess fertility and germline competency, especially in chimeric rodents generated using embryonic stem cells. Traditional methods rely on natural mating and progeny testing, which is time- and cost-intensive. Electroejaculation is a faster method of collecting sperm for genetic analysis and offers the additional benefit of using fewer animals. This column describes a refined electroejaculation technique for chimeric rats using light gas anesthesia and a custom-made platform for sperm collection.

Original language	English (US)
Pages (from-to)	203-205
Number of pages	3
Journal	Lab Animal
Volume	42
Issue number	6
DOIs	https://doi.org/10.1038/laban.301
State	Published - Jun 2013

ASJC Scopus subject areas

Animal Science and Zoology
General Veterinary

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10.1038/laban.301

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title = "Electroejaculation of chimeric rats",

abstract = "With the advent of genetic engineering of rodents came the need to assess fertility and germline competency, especially in chimeric rodents generated using embryonic stem cells. Traditional methods rely on natural mating and progeny testing, which is time- and cost-intensive. Electroejaculation is a faster method of collecting sperm for genetic analysis and offers the additional benefit of using fewer animals. This column describes a refined electroejaculation technique for chimeric rats using light gas anesthesia and a custom-made platform for sperm collection.",

author = "Mccoy, {Marina R.} and Daniel Montonye and Bryda, {Elizabeth C.}",

note = "Funding Information: We thank Miriam Hankins, Nathan Johnson, Mary Shaw and Brad Uppinghouse for their help with this project. This research was supported by NIH P40 OD011062 (E.C.B.), the American Society for Laboratory Animal Practitioners and the RADIL endowment for the University of Missouri Veterinary Research Scholars Program (D.M.) and University of Missouri Life Sciences Mission Enhancement Funds (M.R.M.).",

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Electroejaculation of chimeric rats

Abstract

ASJC Scopus subject areas

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