Efficacy and toxicity of hydrogen peroxide producing electrochemical bandages in a porcine explant biofilm model

Gretchen Tibbits, Abdelrhman Mohamed, Suzanne Gelston, Laure Flurin, Yash S. Raval, Kerryl Greenwood-Quaintance, Robin Patel, Haluk Beyenal

Research output: Contribution to journalArticlepeer-review


Aims: Effects of H2O2 producing electrochemical-bandages (e-bandages) on methicillin-resistant Staphylococcus aureus colonization and biofilm removal were assessed using a porcine explant biofilm model. Transport of H2O2 produced from the e-bandage into explant tissue and associated potential toxicity were evaluated. Methods and Results: Viable prokaryotic cells from infected explants were quantified after 48 h treatment with e-bandages in three ex vivo S. aureus infection models: (1) reducing colonization, (2) removing young biofilms and (3) removing mature biofilms. H2O2 concentration-depth profiles in explants/biofilms were measured using microelectrodes. Reductions in eukaryotic cell viability of polarized and nonpolarized noninfected explants were compared. e-Bandages effectively reduced S. aureus colonization (p = 0.029) and reduced the viable prokaryotic cell concentrations of young biofilms (p = 0.029) with limited effects on mature biofilms (p > 0.1). H2O2 penetrated biofilms and explants and reduced eukaryotic cell viability by 32–44% compared to nonpolarized explants. Conclusions: H2O2 producing e-bandages were most active when used to reduce colonization and remove young biofilms rather than to remove mature biofilms. Significance and Impact of Study: The described e-bandages reduced S. aureus colonization and young S. aureus biofilms in a porcine explant wound model, supporting their further development as an antibiotic-free alternative for managing biofilm infections.

Original languageEnglish (US)
Pages (from-to)3755-3767
Number of pages13
JournalJournal of Applied Microbiology
Issue number6
StatePublished - Dec 2022


  • Staphylococcus aureus
  • biofilm
  • electroceutical
  • electrochemical bandage
  • hydrogen peroxide
  • porcine explant

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology


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