Effects of the aqueous extract of Schizandra chinensis fruit on ethanol withdrawal-induced anxiety in rats

Yi Yan Wu, Zheng Lin Zhao, Yu Peng Yang, Xu Dong Yang, Eun Young Jang, Nathan D. Schilaty, David M. Hedges, Sang Chan Kim, Il Je Cho, Rong Jie Zhao

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Background We previously demonstrated that the aqueous extract of the Schizandra chinensis fruit (AESC) ameliorated Cd-induced depletion of monoamine neurotransmitters in the brain through antioxidant activity. In the present study, we investigated the effect of AESC on anxiety-like behavior and the levels of norepinephrine and 3-methoxy-4-hydroxy- phenylglycol (a metabolite of norepinephrine) in different brain regions during ethanol withdrawal in rats. Methods Male Sprague-Dawley rats were treated with 3 g/kg of ethanol (20%, w/v) or saline by daily intraperitoneal injection for 28 days followed by three days of withdrawal. During withdrawal, rats were given AESC (100 mg kg -1d-1 or 300 mg kg -1d-1, P.O.) once a day for three days. Thirty minutes after the final dose of AESC, the anxiogenic response was evaluated using an elevated plus maze, and the plasma corticosterone levels were examined by radioimmunoassay. Meanwhile, the concentrations of norepinephrine and 3-methoxy-4-hydroxy-phenylglycol in the hypothalamic paraventricular nucleus and hippocampus were also measured by high performance liquid chromatography. Results Rats undergoing ethanol withdrawal exhibited substantial anxiety-like behavior, which was characterized by both the decrease in time spent in the open arms of the elevated plus maze and the increased level of corticosterone secretion, which were greatly attenuated by doses of AESC in a dose-dependent manner. The high performance liquid chromatography analysis revealed that ethanol withdrawal significantly increased norepinephrine and 3-methoxy-4- hydroxy-phenylglycol levels in the hypothalamic paraventricular nucleus, while not significantly altering them in the hippocampus. Similar to the results from the elevated plus maze test, the AESC significantly inhibited the elevation of norepinephrine and its metabolite in the hypothalamic paraventricular nucleus in a dose-dependent manner. Conclusions These results suggest that AESC attenuates anxiety-like behavior induced by ethanol withdrawal through modulation of the hypothalamic norepinephrine system in the brain.

Original languageEnglish (US)
Pages (from-to)1935-1940
Number of pages6
JournalChinese medical journal
Issue number10
StatePublished - 2014


  • Anxiety
  • Ethanol withdrawal
  • Hippocampus
  • Hypothalamic paraventricular nucleus
  • Norepinephrine
  • Schizandra chinensis fruit

ASJC Scopus subject areas

  • General Medicine


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