@article{f1b3aa8cd0224d9699049deaa41f100c,
title = "Effects of Surgical Angiogenesis on Segmental Bone Reconstruction With Cryopreserved Massive-Structural Allografts in a Porcine Tibia Model",
abstract = "Cryopreserved bone allografts (CBA) used to reconstruct segmental bone defects provide immediate structural stability, but are vulnerable to infection, non-union and late stress fracture as the majority of the allograft remains largely avascular. We sought to improve the bone vascularity and bone formation of CBAs by surgical angiogenesis with an implanted arteriovenous (AV) bundle, using a porcine tibial defect model. Cryopreserved tibial bone allografts were transplanted in swine leukocyte antigen (SLA) mismatched Yucatan minipigs to reconstruct a 3.5 cm segmental tibial defect. A cranial tibial AV-bundle was placed within its intramedullary canal to induce angiogenesis. The AV bundle was patent in eight pigs and ligated in a control group of eight pigs. At 20 weeks neo-angiogenesis was evaluated by micro-angiography. Bone formation was measured by quantitative histomorphometry and micro-computed tomography. Seven of eight AV-bundles in the revascularized group were patent. One had thrombosed due to allograft displacement. Total vascular volume was higher in the revascularized allografts compared to the ligated group (p = 0.015). Revascularized allografts had increased levels of bone formation on the allograft endosteal surface compared to the ligated control group (p = 0.05). Surgical angiogenesis of porcine tibial CBAs by intramedullary implantation of an AV-bundle creates an enhanced autogenous neoangiogenic circulation and accelerates active bone formation on allograft endosteal surfaces.",
keywords = "allograft, angiogenesis, bone, porcine, revascularization",
author = "Visser, {Noortje J.} and Rezaie, {Elisa S.} and Friedrich, {Patricia F.} and Dimitra Kotsougiani and Shin, {Alexander Y.} and Bishop, {Allen T.}",
note = "Funding Information: The authors thank Dennis Hanson from the Biomedical Imaging Resource lab, Teresa Decklever from the Mayo Clinic Small Animal Imaging Core and James Herrick, Robert A. Brown and Donna E. Jewison from the Bone Histomorphometry Laboratory at Mayo Clinic Rochester for their contributions for this work. Research reported in this publication was supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health under Award Number AR49718. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Funding Information: The authors thank Dennis Hanson from the Biomedical Imaging Resource lab, Teresa Decklever from the Mayo Clinic Small Animal Imaging Core and James Herrick, Robert A. Brown and Donna E. Jewison from the Bone Histomorphometry Laboratory at Mayo Clinic Rochester for their contributions for this work. Research reported in this publication was supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health under Award Number AR49718. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Publisher Copyright: {\textcopyright} 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.",
year = "2019",
month = aug,
day = "1",
doi = "10.1002/jor.24318",
language = "English (US)",
volume = "37",
pages = "1698--1708",
journal = "Journal of Orthopaedic Research",
issn = "0736-0266",
publisher = "John Wiley and Sons Inc.",
number = "8",
}