TY - JOUR
T1 - Effects of lung preservation with Euro-Collins and University of Wisconsin solutions on endothelium-dependent relaxations
AU - Strüber, Martin
AU - Ehlers, Klaus A.
AU - Nilsson, Folke N.
AU - Miller, Virginia M.
AU - McGregor, Christopher G.A.
AU - Haverich, Axel
PY - 1997/5
Y1 - 1997/5
N2 - Background. This study compares the effect of lung preservation using flush perfusion of Euro-Collins or University of Wisconsin solution on the pulmonary vascular function of endothelium-dependent and endothelium- independent relaxations. Methods. Rings of canine intrapulmonary arteries were studied after 6 hours of cold ischemia in Euro-Collins or University of Wisconsin preservation solution. Endothelium-dependent and endothelium- independent relaxations were induced in organ chamber experiments. To also study pulmonary resistance vessels, endothelium-dependent relaxations were induced in in vitro perfused intact rabbit lungs. Results. In the organ chamber experiments, a moderate but significant (p < 0.05) reduction in endothelium dependent relaxations were found in the perfused and stored vessels. In perfused rabbit lungs, a decrease in the endothelial response occurred immediately after perfusion with Euro-Collins solution. However, a recovery and overshooting response was found after preservation with either solution and 6 hours of cold ischemia. A significant increase in the sensitivity of smooth muscle cells to nitric oxide was shown in both preparations. Conclusions. Both crystalloid perfusion fluids cause a decrease in endothelial function during the perfusion procedure. In contrast, endothelial function is well preserved during the ischemic time. University of Wisconsin solution induced a higher sensitivity of the vascular smooth muscle to the endothelium-derived relaxing factor nitric oxide. A reduction in pulmonary vascular resistance after University of Wisconsin preservation may be of importance in subsequent clinical lung transplantation.
AB - Background. This study compares the effect of lung preservation using flush perfusion of Euro-Collins or University of Wisconsin solution on the pulmonary vascular function of endothelium-dependent and endothelium- independent relaxations. Methods. Rings of canine intrapulmonary arteries were studied after 6 hours of cold ischemia in Euro-Collins or University of Wisconsin preservation solution. Endothelium-dependent and endothelium- independent relaxations were induced in organ chamber experiments. To also study pulmonary resistance vessels, endothelium-dependent relaxations were induced in in vitro perfused intact rabbit lungs. Results. In the organ chamber experiments, a moderate but significant (p < 0.05) reduction in endothelium dependent relaxations were found in the perfused and stored vessels. In perfused rabbit lungs, a decrease in the endothelial response occurred immediately after perfusion with Euro-Collins solution. However, a recovery and overshooting response was found after preservation with either solution and 6 hours of cold ischemia. A significant increase in the sensitivity of smooth muscle cells to nitric oxide was shown in both preparations. Conclusions. Both crystalloid perfusion fluids cause a decrease in endothelial function during the perfusion procedure. In contrast, endothelial function is well preserved during the ischemic time. University of Wisconsin solution induced a higher sensitivity of the vascular smooth muscle to the endothelium-derived relaxing factor nitric oxide. A reduction in pulmonary vascular resistance after University of Wisconsin preservation may be of importance in subsequent clinical lung transplantation.
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U2 - 10.1016/S0003-4975(97)00215-4
DO - 10.1016/S0003-4975(97)00215-4
M3 - Article
C2 - 9146338
AN - SCOPUS:0031149577
SN - 0003-4975
VL - 63
SP - 1428
EP - 1435
JO - Annals of Thoracic Surgery
JF - Annals of Thoracic Surgery
IS - 5
ER -