Effectiveness of patient-collected swabs for influenza testing

Neelam Dhiman, Rita M. Miller, Janet L. Finley, Matthew D. Sztajnkrycer, David M. Nestler, Andy J. Boggust, Sarah M. Jenkins, Thomas F. Smith, John W. Wilson, Franklin R. Cockerill, Bobbi S. Pritt

Research output: Contribution to journalArticlepeer-review

37 Scopus citations


Objective: To compare the effectiveness of self-collected and health care worker (HCW)-collected nasal swabs for detection of influenza viruses and determine the patients' preference for type of collection. Patients and Methods: We enrolled adult patients presenting with influenzalike illness to the Emergency Department at Mayo Clinic, Rochester, Minnesota, from January 28, 2011, through April 30, 2011. Patients self-collected a midturbinate nasal flocked swab from their right nostril following written instructions. A second swab was then collected by an HCW from the left nostril. Swabs were tested for influenza A and B viruses by real-time reverse transcription-polymerase chain reaction, and percent concordance between collection methods was determined. Results: Of the 72 paired specimens analyzed, 25 were positive for influenza A or B RNA by at least one of the collection methods (34.7% positivity rate). When the 14 patients who had prior health care training were excluded, the qualitative agreement between collection methods was 94.8% (55 of 58). Two of the 58 specimens (3.4%) from patients without health care training were positive only by HCW collection, and 1 of 58 (1.7%) was positive only by patient self-collection. A total of 53.4% of patients (31 of 58) preferred the self-collection method over theHCWcollection, and 25.9% (15 of 58) had no preference. Conclusion: Self-collected midturbinate nasal swabs provide a reliable alternative to HCW collection for influenza A and B virus real-time reverse transcription-polymerase chain reaction.

Original languageEnglish (US)
Pages (from-to)548-554
Number of pages7
JournalMayo Clinic proceedings
Issue number6
StatePublished - Jun 2012

ASJC Scopus subject areas

  • General Medicine


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