TY - JOUR
T1 - Effect of the Artificial Sweetener, Acesulfame Potassium, a Sweet Taste Receptor Agonist, on Glucose Uptake in Small Intestinal Cell Lines
AU - Zheng, Ye
AU - Sarr, Michael G.
N1 - Funding Information:
This work was supported in part by NIH grant R01 DK039337 (MGS).
PY - 2013/1
Y1 - 2013/1
N2 - Introduction: Sweet taste receptors may enhance glucose absorption. Aim: This study aimed to explore the cell biology of sweet taste receptors on glucose uptake. Hypothesis: Artificial sweeteners increase glucose uptake via activating sweet taste receptors in the enterocyte to translocate GLUT2 to the apical membrane through the PLC βII pathway. Methods: Caco-2, RIE-1, and IEC-6 cells, starved from glucose for 1 h were pre-incubated with 10 mM acesulfame potassium (AceK). Glucose uptake was measured by incubating cells for 1 to 10 min with 0. 5-50 mM glucose with or without U-73122, chelerythrine, and cytochalasin B. Results: In Caco-2 and RIE-1 cells, 10 mM AceK increased glucose uptake by 20-30 % at glucose >25 mM, but not in lesser glucose concentrations (<10 mM), nor at 1 min or 10 min incubations. U-73122 (PLC βII inhibitor) inhibited uptake at glucose >25 mM and for 5 min incubation; chelerythrine and cytochalasin B had similar effects. No effect occurred in IEC-6 cells. Summary: Activation of sweet taste receptors had no effect on glucose uptake in low (<25 mM) glucose concentrations but increased uptake at greater concentrations (>25 mM). Conclusions: Role of artificial sweeteners on glucose uptake appears to act in part by effects on the enterocyte itself.
AB - Introduction: Sweet taste receptors may enhance glucose absorption. Aim: This study aimed to explore the cell biology of sweet taste receptors on glucose uptake. Hypothesis: Artificial sweeteners increase glucose uptake via activating sweet taste receptors in the enterocyte to translocate GLUT2 to the apical membrane through the PLC βII pathway. Methods: Caco-2, RIE-1, and IEC-6 cells, starved from glucose for 1 h were pre-incubated with 10 mM acesulfame potassium (AceK). Glucose uptake was measured by incubating cells for 1 to 10 min with 0. 5-50 mM glucose with or without U-73122, chelerythrine, and cytochalasin B. Results: In Caco-2 and RIE-1 cells, 10 mM AceK increased glucose uptake by 20-30 % at glucose >25 mM, but not in lesser glucose concentrations (<10 mM), nor at 1 min or 10 min incubations. U-73122 (PLC βII inhibitor) inhibited uptake at glucose >25 mM and for 5 min incubation; chelerythrine and cytochalasin B had similar effects. No effect occurred in IEC-6 cells. Summary: Activation of sweet taste receptors had no effect on glucose uptake in low (<25 mM) glucose concentrations but increased uptake at greater concentrations (>25 mM). Conclusions: Role of artificial sweeteners on glucose uptake appears to act in part by effects on the enterocyte itself.
KW - Acesulfame potassium
KW - GLUT2
KW - PLC βII
KW - Sweet taste receptor
UR - http://www.scopus.com/inward/record.url?scp=84871994868&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84871994868&partnerID=8YFLogxK
U2 - 10.1007/s11605-012-1998-z
DO - 10.1007/s11605-012-1998-z
M3 - Article
C2 - 22948835
AN - SCOPUS:84871994868
SN - 1091-255X
VL - 17
SP - 153
EP - 158
JO - Journal of Gastrointestinal Surgery
JF - Journal of Gastrointestinal Surgery
IS - 1
ER -