Effect of Some Calcium Antagonists on Muscarinic Receptor‐Mediated Cyclic GMP Formation

Esam El‐Fakahany, Elliott Richelson

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Abstract: Several calcium antagonists were screened for their effect on muscarinic acetylcholine receptor‐mediated cyclic GMP formation in murine neuroblastoma cells (clone N1E‐115). Mn2+, Ni2+, and verapamil rapidly antagonized the response noncompetitively, with the following order of potency: verapamil > Mn2+ > Ni2+. The effects of Mn2+ and Ni2+, but not those of verapamil, were largely reversed by increasing extracellular calcium concentration. Additional effects of these agents included displacement of [3H]quinuclidinyl benzilate binding by verapamil and elevation of cyclic GMP levels by Mn2+ and Ni2+ in the absence of agonists. These results are in support of the hypothesis that receptor‐mediated cyclic GMP formation by these cells is dependent upon entry of calcium into the cell and demonstrate the complexity of the effects of calcium antagonists.

Original languageEnglish (US)
Pages (from-to)705-710
Number of pages6
JournalJournal of neurochemistry
Issue number3
StatePublished - Mar 1983


  • Calcium antagonists
  • Carbamylcholine
  • Cyclic GMP
  • Manganese ions
  • Nickel ions
  • Verapamil

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Biochemistry


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