Effect of residual platelets in frozen-thawed plasma on results of dilute Russell’s viper venom time assay for lupus anticoagulant testing

Brenda J. Nelson Strande; Meera Sridharan; Rachel R. Leger; Melissa S. Stuart; Julie I. Tange; Serena D. Navitska; Nahla Heikal; Aneel A. Ashrani; Dong Chen; Jansen N. Seheult; Rajiv K. Pruthi

doi:10.1093/ajcp/aqad138

Effect of residual platelets in frozen-thawed plasma on results of dilute Russell’s viper venom time assay for lupus anticoagulant testing

Brenda J. Nelson Strande, Meera Sridharan, Rachel R. Leger, Melissa S. Stuart, Julie I. Tange, Serena D. Navitska, Nahla Heikal, Aneel A. Ashrani, Dong Chen, Jansen N. Seheult, Rajiv K. Pruthi

Hematology

Research output: Contribution to journal › Article › peer-review

Abstract

Objectives: To determine the impact of residual platelets on dilute Russell’s viper venom time (DRVVT) assay in frozen-thawed plasma submitted for lupus anticoagulant (LAC) testing. Methods: We measured platelet counts in frozen-thawed samples submitted for LAC testing and evaluated the association between platelet count and the DRVVT screening time and ratios. We also spiked platelets into a LAC-positive sample to observe the effect on the DRVVT. Results: Progressive increase in platelet count resulted in a statistically significant shortening of the DRVVT assay results on plasma after 1 freeze-thaw cycle. A similar effect was noted on the LAC-positive sample. Conclusions: Residual platelets in plasma samples result in shortening of DRVVT assay after 1 freeze-thaw cycle. This may result in a false-negative LAC test result.

Original language	English (US)
Pages (from-to)	212-215
Number of pages	4
Journal	American journal of clinical pathology
Volume	161
Issue number	3
DOIs	https://doi.org/10.1093/ajcp/aqad138
State	Published - Mar 1 2024

Keywords

dilute Russell’s viper venom time
freeze-thaw
lupus anticoagulant (LAC)
platelet-poor plasma

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1093/ajcp/aqad138

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Nelson Strande, B. J., Sridharan, M., Leger, R. R., Stuart, M. S., Tange, J. I., Navitska, S. D., Heikal, N., Ashrani, A. A., Chen, D., Seheult, J. N., & Pruthi, R. K. (2024). Effect of residual platelets in frozen-thawed plasma on results of dilute Russell’s viper venom time assay for lupus anticoagulant testing. American journal of clinical pathology, 161(3), 212-215. https://doi.org/10.1093/ajcp/aqad138

Nelson Strande, BJ, Sridharan, M, Leger, RR, Stuart, MS, Tange, JI, Navitska, SD, Heikal, N, Ashrani, AA, Chen, D, Seheult, JN & Pruthi, RK 2024, 'Effect of residual platelets in frozen-thawed plasma on results of dilute Russell’s viper venom time assay for lupus anticoagulant testing', American journal of clinical pathology, vol. 161, no. 3, pp. 212-215. https://doi.org/10.1093/ajcp/aqad138

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title = "Effect of residual platelets in frozen-thawed plasma on results of dilute Russell{\textquoteright}s viper venom time assay for lupus anticoagulant testing",

abstract = "Objectives: To determine the impact of residual platelets on dilute Russell{\textquoteright}s viper venom time (DRVVT) assay in frozen-thawed plasma submitted for lupus anticoagulant (LAC) testing. Methods: We measured platelet counts in frozen-thawed samples submitted for LAC testing and evaluated the association between platelet count and the DRVVT screening time and ratios. We also spiked platelets into a LAC-positive sample to observe the effect on the DRVVT. Results: Progressive increase in platelet count resulted in a statistically significant shortening of the DRVVT assay results on plasma after 1 freeze-thaw cycle. A similar effect was noted on the LAC-positive sample. Conclusions: Residual platelets in plasma samples result in shortening of DRVVT assay after 1 freeze-thaw cycle. This may result in a false-negative LAC test result.",

keywords = "dilute Russell{\textquoteright}s viper venom time, freeze-thaw, lupus anticoagulant (LAC), platelet-poor plasma",

author = "{Nelson Strande}, {Brenda J.} and Meera Sridharan and Leger, {Rachel R.} and Stuart, {Melissa S.} and Tange, {Julie I.} and Navitska, {Serena D.} and Nahla Heikal and Ashrani, {Aneel A.} and Dong Chen and Seheult, {Jansen N.} and Pruthi, {Rajiv K.}",

year = "2024",

month = mar,

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doi = "10.1093/ajcp/aqad138",

language = "English (US)",

volume = "161",

pages = "212--215",

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T1 - Effect of residual platelets in frozen-thawed plasma on results of dilute Russell’s viper venom time assay for lupus anticoagulant testing

AU - Nelson Strande, Brenda J.

AU - Sridharan, Meera

AU - Leger, Rachel R.

AU - Stuart, Melissa S.

AU - Tange, Julie I.

AU - Navitska, Serena D.

AU - Heikal, Nahla

AU - Ashrani, Aneel A.

AU - Chen, Dong

AU - Seheult, Jansen N.

AU - Pruthi, Rajiv K.

PY - 2024/3/1

Y1 - 2024/3/1

N2 - Objectives: To determine the impact of residual platelets on dilute Russell’s viper venom time (DRVVT) assay in frozen-thawed plasma submitted for lupus anticoagulant (LAC) testing. Methods: We measured platelet counts in frozen-thawed samples submitted for LAC testing and evaluated the association between platelet count and the DRVVT screening time and ratios. We also spiked platelets into a LAC-positive sample to observe the effect on the DRVVT. Results: Progressive increase in platelet count resulted in a statistically significant shortening of the DRVVT assay results on plasma after 1 freeze-thaw cycle. A similar effect was noted on the LAC-positive sample. Conclusions: Residual platelets in plasma samples result in shortening of DRVVT assay after 1 freeze-thaw cycle. This may result in a false-negative LAC test result.

AB - Objectives: To determine the impact of residual platelets on dilute Russell’s viper venom time (DRVVT) assay in frozen-thawed plasma submitted for lupus anticoagulant (LAC) testing. Methods: We measured platelet counts in frozen-thawed samples submitted for LAC testing and evaluated the association between platelet count and the DRVVT screening time and ratios. We also spiked platelets into a LAC-positive sample to observe the effect on the DRVVT. Results: Progressive increase in platelet count resulted in a statistically significant shortening of the DRVVT assay results on plasma after 1 freeze-thaw cycle. A similar effect was noted on the LAC-positive sample. Conclusions: Residual platelets in plasma samples result in shortening of DRVVT assay after 1 freeze-thaw cycle. This may result in a false-negative LAC test result.

KW - dilute Russell’s viper venom time

KW - freeze-thaw

KW - lupus anticoagulant (LAC)

KW - platelet-poor plasma

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Effect of residual platelets in frozen-thawed plasma on results of dilute Russell’s viper venom time assay for lupus anticoagulant testing

Abstract

Keywords

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