TY - JOUR
T1 - Effect of multiple freeze-thaw cycles on detection of measles, mumps, and rubella virus antibodies
AU - Pinsky, Norman A.
AU - Huddleston, Jeanne M.
AU - Jacobson, Robert M.
AU - Wollan, Peter C.
AU - Poland, Gregory A.
PY - 2003/1
Y1 - 2003/1
N2 - We investigated the effect of multiple freeze-thaw cycles on mumps, measles, and rubella virus serum antibody levels with whole-virus immunoglobulin G enzyme-linked immunoassays. Fresh serum samples from nine healthy adult volunteers were divided into six sets of five aliquots each. Samples were taken through a total of 10 freeze-thaw cycles and stored at 4°C until assayed. Each assay measurement was done in replicates of five, and the mean value was reported. After completing 10 freeze-thaw cycles, we found no clinically or statistically significant effect on measured antibody levels and found no discernible detrimental effect on the ability to measure these antibodies by enzyme-linked immunoassays.
AB - We investigated the effect of multiple freeze-thaw cycles on mumps, measles, and rubella virus serum antibody levels with whole-virus immunoglobulin G enzyme-linked immunoassays. Fresh serum samples from nine healthy adult volunteers were divided into six sets of five aliquots each. Samples were taken through a total of 10 freeze-thaw cycles and stored at 4°C until assayed. Each assay measurement was done in replicates of five, and the mean value was reported. After completing 10 freeze-thaw cycles, we found no clinically or statistically significant effect on measured antibody levels and found no discernible detrimental effect on the ability to measure these antibodies by enzyme-linked immunoassays.
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U2 - 10.1128/CDLI.10.1.19-21.2003
DO - 10.1128/CDLI.10.1.19-21.2003
M3 - Article
C2 - 12522034
AN - SCOPUS:0037249542
SN - 1071-412X
VL - 10
SP - 19
EP - 21
JO - Clinical and Diagnostic Laboratory Immunology
JF - Clinical and Diagnostic Laboratory Immunology
IS - 1
ER -