Effect of functionalized micropatterned PLGA on guided neurite growth

Li Yao; Shenguo Wang; Wenjin Cui; Richard Sherlock; Claire O'Connell; Gopinath Damodaran; Adrienne Gorman; Anthony Windebank; Abhay Pandit

doi:10.1016/j.actbio.2008.09.002

Effect of functionalized micropatterned PLGA on guided neurite growth

Li Yao, Shenguo Wang, Wenjin Cui, Richard Sherlock, Claire O'Connell, Gopinath Damodaran, Adrienne Gorman, Anthony Windebank, Abhay Pandit

Neurology

Research output: Contribution to journal › Article › peer-review

112 Scopus citations

Abstract

When coaptation is not possible in the repair of nerve injuries, a bridge of biomaterial scaffold provides a structural support for neuronal cell growth and guides nerve regeneration. Poly(lactide-co-glycolide) (PLGA) scaffolds have been widely investigated for neural tissue engineering applications. In order to investigate guided neurite growth, we have fabricated micropatterns on PLGA films using laser ablation methods. The micropatterned PLGA films were coated with collagen type I or laminin peptide (PPFLMLLKGSTR) to promote axon growth. Micropatterned PLGA films provide a guidance effect on both early stage neurite outgrowth and elongation. Small (5 μm) grooves showed more statistically significant parallel neurite growth compared with larger size grooves (10 μm). Micropatterned PLGA films coated with laminin peptide showed more parallel neurite growth compared with those coated with collagen type I. Primary neurite number and total neurite length per cell decreased on micropatterned PLGA films compared with the controls. Neurites showed a preference for growth in the microgrooves rather than on the spaces. This study indicates that surface micropatterned structures with conjugated functional molecules can be used to guide neurite growth.

Original language	English (US)
Pages (from-to)	580-588
Number of pages	9
Journal	Acta Biomaterialia
Volume	5
Issue number	2
DOIs	https://doi.org/10.1016/j.actbio.2008.09.002
State	Published - Feb 2009

Keywords

Guided regeneration
Laminin peptide
Micropatterning
PLGA

ASJC Scopus subject areas

Biotechnology
Biomaterials
Biochemistry
Biomedical Engineering
Molecular Biology

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10.1016/j.actbio.2008.09.002

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title = "Effect of functionalized micropatterned PLGA on guided neurite growth",

abstract = "When coaptation is not possible in the repair of nerve injuries, a bridge of biomaterial scaffold provides a structural support for neuronal cell growth and guides nerve regeneration. Poly(lactide-co-glycolide) (PLGA) scaffolds have been widely investigated for neural tissue engineering applications. In order to investigate guided neurite growth, we have fabricated micropatterns on PLGA films using laser ablation methods. The micropatterned PLGA films were coated with collagen type I or laminin peptide (PPFLMLLKGSTR) to promote axon growth. Micropatterned PLGA films provide a guidance effect on both early stage neurite outgrowth and elongation. Small (5 μm) grooves showed more statistically significant parallel neurite growth compared with larger size grooves (10 μm). Micropatterned PLGA films coated with laminin peptide showed more parallel neurite growth compared with those coated with collagen type I. Primary neurite number and total neurite length per cell decreased on micropatterned PLGA films compared with the controls. Neurites showed a preference for growth in the microgrooves rather than on the spaces. This study indicates that surface micropatterned structures with conjugated functional molecules can be used to guide neurite growth.",

keywords = "Guided regeneration, Laminin peptide, Micropatterning, PLGA",

author = "Li Yao and Shenguo Wang and Wenjin Cui and Richard Sherlock and Claire O'Connell and Gopinath Damodaran and Adrienne Gorman and Anthony Windebank and Abhay Pandit",

note = "Funding Information: We acknowledge China/Ireland Science and Technology Collaboration Research Fund Joint Project, Science Foundation Ireland (SFI) & Ministry of Science and Technology People{\textquoteright}s Republic of China (MOST), Dystrophic Epidermal Bullosa Research Association, European FP6 – Marie Curie TOK Award and Major State Basic Research Development Program of China (No. 2005CB5227074) for providing funding for this project.",

year = "2009",

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doi = "10.1016/j.actbio.2008.09.002",

language = "English (US)",

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AU - Yao, Li

AU - Wang, Shenguo

AU - Cui, Wenjin

AU - Sherlock, Richard

AU - O'Connell, Claire

AU - Damodaran, Gopinath

AU - Gorman, Adrienne

AU - Windebank, Anthony

AU - Pandit, Abhay

N1 - Funding Information: We acknowledge China/Ireland Science and Technology Collaboration Research Fund Joint Project, Science Foundation Ireland (SFI) & Ministry of Science and Technology People’s Republic of China (MOST), Dystrophic Epidermal Bullosa Research Association, European FP6 – Marie Curie TOK Award and Major State Basic Research Development Program of China (No. 2005CB5227074) for providing funding for this project.

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N2 - When coaptation is not possible in the repair of nerve injuries, a bridge of biomaterial scaffold provides a structural support for neuronal cell growth and guides nerve regeneration. Poly(lactide-co-glycolide) (PLGA) scaffolds have been widely investigated for neural tissue engineering applications. In order to investigate guided neurite growth, we have fabricated micropatterns on PLGA films using laser ablation methods. The micropatterned PLGA films were coated with collagen type I or laminin peptide (PPFLMLLKGSTR) to promote axon growth. Micropatterned PLGA films provide a guidance effect on both early stage neurite outgrowth and elongation. Small (5 μm) grooves showed more statistically significant parallel neurite growth compared with larger size grooves (10 μm). Micropatterned PLGA films coated with laminin peptide showed more parallel neurite growth compared with those coated with collagen type I. Primary neurite number and total neurite length per cell decreased on micropatterned PLGA films compared with the controls. Neurites showed a preference for growth in the microgrooves rather than on the spaces. This study indicates that surface micropatterned structures with conjugated functional molecules can be used to guide neurite growth.

AB - When coaptation is not possible in the repair of nerve injuries, a bridge of biomaterial scaffold provides a structural support for neuronal cell growth and guides nerve regeneration. Poly(lactide-co-glycolide) (PLGA) scaffolds have been widely investigated for neural tissue engineering applications. In order to investigate guided neurite growth, we have fabricated micropatterns on PLGA films using laser ablation methods. The micropatterned PLGA films were coated with collagen type I or laminin peptide (PPFLMLLKGSTR) to promote axon growth. Micropatterned PLGA films provide a guidance effect on both early stage neurite outgrowth and elongation. Small (5 μm) grooves showed more statistically significant parallel neurite growth compared with larger size grooves (10 μm). Micropatterned PLGA films coated with laminin peptide showed more parallel neurite growth compared with those coated with collagen type I. Primary neurite number and total neurite length per cell decreased on micropatterned PLGA films compared with the controls. Neurites showed a preference for growth in the microgrooves rather than on the spaces. This study indicates that surface micropatterned structures with conjugated functional molecules can be used to guide neurite growth.

KW - Guided regeneration

KW - Laminin peptide

KW - Micropatterning

KW - PLGA

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Effect of functionalized micropatterned PLGA on guided neurite growth

Abstract

Keywords

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