Effect of fibrillation on acetylcholinesterase mRNA in cultured embryonic rat myotubes

L. H. Younkin; C. F. McTiernan; S. G. Younkin

doi:10.1016/0014-4827(88)90161-9

Effect of fibrillation on acetylcholinesterase mRNA in cultured embryonic rat myotubes

L. H. Younkin, C. F. McTiernan, S. G. Younkin

Neuroscience

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Acetylcholinesterase (AChE) and AChE mRNA were evaluated in spontaneously fibrillating myotubes derived from 20-day-old rat fetuses and in matched cultures in which fibrillation was prevented by adding tetrodotoxin on the fourth day of culture. On the eighth day of culture, the AChE activity of fibrillating and nonfibrillating cultures was 5332 and 1861 pmol ACh hydrolyzed min^-1 dish^-1, respectively (P < 0.005). Total mRNA was essentially the same in fibrillating and nonfibrillating cultures (27.4 and 25.4 μg/dish, respectively). AChE mRNA was assessed by assaying the AChE produced by Xenopus oocytes microinjected with purified mRNA. The AChE produced by mRNA from fibrillating and nonfibrillating cultures was 0.46 and 0.10 pmol ACh hydrolyzed min^-1 oocyte^-1, respectively (P < 0.005).

Original language	English (US)
Pages (from-to)	279-281
Number of pages	3
Journal	Experimental Cell Research
Volume	174
Issue number	1
DOIs	https://doi.org/10.1016/0014-4827(88)90161-9
State	Published - Jan 1988

ASJC Scopus subject areas

Cell Biology

Access to Document

10.1016/0014-4827(88)90161-9

Cite this

@article{a3e9693eb31747bdb5beae578ab86f6c,

title = "Effect of fibrillation on acetylcholinesterase mRNA in cultured embryonic rat myotubes",

abstract = "Acetylcholinesterase (AChE) and AChE mRNA were evaluated in spontaneously fibrillating myotubes derived from 20-day-old rat fetuses and in matched cultures in which fibrillation was prevented by adding tetrodotoxin on the fourth day of culture. On the eighth day of culture, the AChE activity of fibrillating and nonfibrillating cultures was 5332 and 1861 pmol ACh hydrolyzed min-1 dish-1, respectively (P < 0.005). Total mRNA was essentially the same in fibrillating and nonfibrillating cultures (27.4 and 25.4 μg/dish, respectively). AChE mRNA was assessed by assaying the AChE produced by Xenopus oocytes microinjected with purified mRNA. The AChE produced by mRNA from fibrillating and nonfibrillating cultures was 0.46 and 0.10 pmol ACh hydrolyzed min-1 oocyte-1, respectively (P < 0.005).",

author = "Younkin, {L. H.} and McTiernan, {C. F.} and Younkin, {S. G.}",

year = "1988",

month = jan,

doi = "10.1016/0014-4827(88)90161-9",

language = "English (US)",

volume = "174",

pages = "279--281",

journal = "Experimental Cell Research",

issn = "0014-4827",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Effect of fibrillation on acetylcholinesterase mRNA in cultured embryonic rat myotubes

AU - Younkin, L. H.

AU - McTiernan, C. F.

AU - Younkin, S. G.

PY - 1988/1

Y1 - 1988/1

N2 - Acetylcholinesterase (AChE) and AChE mRNA were evaluated in spontaneously fibrillating myotubes derived from 20-day-old rat fetuses and in matched cultures in which fibrillation was prevented by adding tetrodotoxin on the fourth day of culture. On the eighth day of culture, the AChE activity of fibrillating and nonfibrillating cultures was 5332 and 1861 pmol ACh hydrolyzed min-1 dish-1, respectively (P < 0.005). Total mRNA was essentially the same in fibrillating and nonfibrillating cultures (27.4 and 25.4 μg/dish, respectively). AChE mRNA was assessed by assaying the AChE produced by Xenopus oocytes microinjected with purified mRNA. The AChE produced by mRNA from fibrillating and nonfibrillating cultures was 0.46 and 0.10 pmol ACh hydrolyzed min-1 oocyte-1, respectively (P < 0.005).

AB - Acetylcholinesterase (AChE) and AChE mRNA were evaluated in spontaneously fibrillating myotubes derived from 20-day-old rat fetuses and in matched cultures in which fibrillation was prevented by adding tetrodotoxin on the fourth day of culture. On the eighth day of culture, the AChE activity of fibrillating and nonfibrillating cultures was 5332 and 1861 pmol ACh hydrolyzed min-1 dish-1, respectively (P < 0.005). Total mRNA was essentially the same in fibrillating and nonfibrillating cultures (27.4 and 25.4 μg/dish, respectively). AChE mRNA was assessed by assaying the AChE produced by Xenopus oocytes microinjected with purified mRNA. The AChE produced by mRNA from fibrillating and nonfibrillating cultures was 0.46 and 0.10 pmol ACh hydrolyzed min-1 oocyte-1, respectively (P < 0.005).

UR - http://www.scopus.com/inward/record.url?scp=0023871881&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023871881&partnerID=8YFLogxK

U2 - 10.1016/0014-4827(88)90161-9

DO - 10.1016/0014-4827(88)90161-9

M3 - Article

C2 - 3335227

AN - SCOPUS:0023871881

SN - 0014-4827

VL - 174

SP - 279

EP - 281

JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 1

ER -

Effect of fibrillation on acetylcholinesterase mRNA in cultured embryonic rat myotubes

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this