TY - JOUR
T1 - Dual TCR-Expressing T Cells in Cancer
T2 - How Single-Cell Technologies Enable New Investigation
AU - Muhowski, Elizabeth M.
AU - Rogers, Laura M.
N1 - Publisher Copyright:
© 2023 The Authors.
PY - 2023/5
Y1 - 2023/5
N2 - TCR diversity measures are often used to understand the immune response in cancer. Traditional measures of diversity rely on bulk RNA sequencing (RNAseq) of the β-chain variable regions. However, the full αβ TCR repertoire is a combination of both the α- and β-chains, which are encoded by separate genes. In contrast with bulk RNAseq, single-cell RNAseq (scRNAseq) allows paired chain analyses, yielding a more accurate measure of the repertoire. Interestingly, ~30% of mature peripheral T cells express multiple TCR alleles (e.g., two α-chains) and may exhibit dual Ag specificity. scRNAseq has become increasingly common, and data from both human and animal studies are publicly available. However, routine workflows discard secondary TCR alleles and focus on a single TCR clone per cell. This perspectives piece emphasizes why this may not be good practice and highlights unanswered questions in the field of T cell dual specificity.
AB - TCR diversity measures are often used to understand the immune response in cancer. Traditional measures of diversity rely on bulk RNA sequencing (RNAseq) of the β-chain variable regions. However, the full αβ TCR repertoire is a combination of both the α- and β-chains, which are encoded by separate genes. In contrast with bulk RNAseq, single-cell RNAseq (scRNAseq) allows paired chain analyses, yielding a more accurate measure of the repertoire. Interestingly, ~30% of mature peripheral T cells express multiple TCR alleles (e.g., two α-chains) and may exhibit dual Ag specificity. scRNAseq has become increasingly common, and data from both human and animal studies are publicly available. However, routine workflows discard secondary TCR alleles and focus on a single TCR clone per cell. This perspectives piece emphasizes why this may not be good practice and highlights unanswered questions in the field of T cell dual specificity.
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U2 - 10.4049/immunohorizons.2200062
DO - 10.4049/immunohorizons.2200062
M3 - Article
C2 - 37129560
AN - SCOPUS:85159542772
SN - 2573-7732
VL - 7
SP - 299
EP - 306
JO - ImmunoHorizons
JF - ImmunoHorizons
IS - 5
ER -